A simple method based on sputtering and electrochemical deposition onto an anodic aluminum oxide template is presented to fabricate an ordered nanoporous Fe₂O₃/Au film. Investigation results indicated that the prepared porous film consisted of an ordered hexagonal array of nanoholes with a pore diameter of 50 nm and a periodic distance of 120 nm. After dissolving the template, the independent Fe₂O₃/Au film can be transferred onto an ITO substrate to be used as an effective non-enzymatic sensor for detection of ascorbic acid. It exhibited excellent electrocatalytic performance with a high sensitivity of 1281.9 μA mM⁻¹ cm⁻², a wide linear range of 25 μM to 10 mM, and a low detection limit of 1 μM. The satisfactory results obtained indicated that the proposed sensor was promising for the development of a novel strategy for AA detection.