Contact angle changes induced by immunocomplex formation†
Jong-Hoon Kim,Amy Q. Shen,Kyong-Hoon Lee,Gerard A. Cangelosi,Jae-Hyun Chung
Analyst Pub Date : 01/08/2014 00:00:00 , DOI:10.1039/C3AN02189K
Abstract

Immunoassays analyzing interactions between antigens and antibodies can be affected by capillary action together with binding affinity. This paper studies contact-angle changes of bacterial suspensions on antibody immobilized surfaces. The capillary action and the dried pattern of the bacterial suspensions are analyzed and correlated with specific- and nonspecific bindings between bacteria and antibodies.

Graphical abstract: Contact angle changes induced by immunocomplex formation