An efficient green approach for the biocatalytic epoxidation of fatty compounds derived from soyadeodistillate using Fermase CalB enzyme with hydrogen peroxide under acid free conditions is described. To the best of our knowledge this is the first report on the chemoenzymatic epoxidation of fatty esters having branched alkyl chain without using additional acid as an oxygen carrier. After completion of the reaction the immobilized enzyme catalyst was easily recovered by filtration and reused for successive four runs with the marginal loss in catalytic activity.