A fingerprint analysis method was developed for the quality control of Salvia yunnanensis roots by HPLC. The HPLC separation was performed on a Hypersil ODS (4.6 mm × 250 mm, 5 μm) column by gradient elution with acetonitrile–water as the mobile phase, at the temperature of 30 °C and a flow rate of 1.0 mL min−1. A satisfactory separation of 21 common peaks was achieved within 60 min. Similarity evaluation was performed by professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine, and the similarity among 11 batches of samples was no less than 0.92. The results of similarity evaluation confirmed that the quality of Salvia yunnanensis roots was stable, and the developed fingerprint analysis was precise and efficient for the quality control of Salvia yunnanensis roots.
