2. Epigenetics
  3. Protein Arginine Deiminase
  4. Adargiminase

Adargiminase (NEI-01) 是一种经修饰的精氨酸耗竭酶和白蛋白结合剂。Adargiminase 可催化精氨酸转化为瓜氨酸和氨，将血浆精氨酸水平降至检测不到的程度，并可结合小家鼠 (小鼠)、褐家鼠 (大鼠)、家犬 (犬) 和智人 (人类) 的血清白蛋白以延长半衰期。Adargiminase 可抑制 ASS1 阴性胰腺癌细胞的活力，降低肿瘤体积和重量。Adargiminase 可用于胰腺癌的研究。

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Adargiminase

Adargiminase Chemical Structure

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Adargiminase 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Adargiminase (NEI-01) is a modified arginine-depleting enzyme and albumin binder. Adargiminase catalyzes the conversion of arginine to citrulline and ammonia, reduces plasma arginine levels to undetectable levels, and binds to serum albumin from Mus musculus (mouse), Rattus norvegicus (rat), Canis lupus familiaris (dog) and Homo sapiens (human) to extend its half-life. Adargiminase inhibits the viability of ASS1-negative pancreatic cancer cells, and reduces tumor volume and weight. Adargiminase can be used for the research of pancreatic cancer[1].

体外研究
(In Vitro)

Adargiminase (0.001-10 μg/mL; 72 h) 可强效抑制 ASS1 阴性的 MIA PaCa-2 和 PANC-1 胰腺癌细胞的活力，但对 ASS1 阳性的 SMMC7721 肝癌细胞无抑制作用[1]。
Adargiminase (0.1 μg/mL; 6-96 h) 可诱导 MIA PaCa-2 胰腺癌细胞中自噬通路激活 (LC3-II、Beclin 1、磷酸化 AMPKα 水平升高) 及凋亡通路激活 (PARP-1 水平降低)[1]。
Adargiminase (0.001-1 μg/mL; 72 h) 对适配 NEI-01 的 MIA PaCa-2 胰腺癌细胞无作用，但在不含 NEI-01 的培养基中培养 96 h 后，可使这些细胞重新致敏[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Adargiminase 相关抗体:

Cell Viability Assay[1]

Cell Line: Human pancreatic cancer cell lines (MIA PaCa-2, PANC-1), human hepatocellular carcinoma cell line (SMMC7721)
Concentration: 0.001-10 μg/mL
Incubation Time: 72 h
Result: Inhibited viability of ASS1-negative MIA PaCa-2 cells with an IC50 of 0.086 μg/mL.
Inhibited viability of ASS1-negative PANC-1 cells with an IC50 of 0.190 μg/mL.
Showed no significant inhibition of ASS1-positive SMMC7721 cells, with an IC50 >10 μg/mL.

Western Blot Analysis[1]

Cell Line: MIA PaCa-2 human pancreatic cancer cell line
Concentration: 0.1 μg/mL
Incubation Time: 6-96 h
Result: Increased levels of LC3-II, Beclin 1, and phospho-AMPKα.
Decreased levels of PARP-1.
Indicated activation of both autophagic and apoptotic pathways.

Cell Viability Assay[1]

Cell Line: NEI-01-adapted and washout MIA PaCa-2 human pancreatic cancer cell line
Concentration: 0.001-1 μg/mL
Incubation Time: 72 h
Result: Showed resistance to NEI-01 treatment in NEI-01-adapted MIA PaCa-2 cells.
Resensitized washout MIA PaCa-2 cells to NEI-01 with an IC50 of 0.012 μg/mL.
体内研究
(In Vivo)

Adargiminase (腹腔注射；2-5 U；每周 2 次) 可显著降低小鼠异种移植模型中的胰腺肿瘤体积和重量[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c female athymic nude mice (4-6-week-old; pancreatic cancer xenograft model)[1]
Dosage: 2 U; 5 U
Administration: i.p.; twice a week
Result: Significantly reduced tumor volume compared to control (2 U dose).
Significantly reduced tumor volume (p < 0.01) and tumor weight (p = 0.004) compared to control (5 U dose).
Reduced plasma arginine levels over the course of the experiment.
Induced only a very slight increase in ASS1 expression in tumor tissue.
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
组分

Please refer to the lot-specific COA for specific buffer information.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Adargiminase 相关分类

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  • 稀释计算器

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× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AdargiminaseNEI-01NEI01NEI 01Protein Arginine DeiminasePeptidylarginine Deiminasehuman pancreatic mouse xenograft modelsdog serum albuminrat serum albuminASS1-negative pancreatic cancer cellsmouse serum albuminMIA PaCa-2 pancreatic cancer cellsautophagic pathwaysASS1-positive SMMC7721 hepatocellular carcinoma cellsapoptotic pathwaysPANC-1 pancreatic cancer cellsInhibitorinhibitorinhibit

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