1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. Fas Ligand 抗体 (YA4042)

Fas Ligand Antibody (YA4042) 是一个小鼠来源、无偶联标记、抗 Fas Ligand 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥609 In-stock
50 μL ¥1575 In-stock
100 μL ¥2520 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Fas Ligand Antibody (YA4042) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Fas Ligand.

宿主

Mouse

克隆性

Monoclonal Antibody

分子量
Predicted band size: 31 kDa;
Observed band size: 31 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

Purified recombinant fragment of human CD178 (AA: extra 103-281) expressed in E. Coli.

应用 & 推荐
稀释比例
应用 稀释比
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
FC
FC: 流式细胞术
1:200-1:400
ELISA
ELISA: 酶联免疫吸附试验
1:10000
纯度 affinity purified. 偶联 Non-conjugated
修饰 Unmodified 同型 IgG1
性状

液体

组分

Supplied in PBS with 0.05% sodium azide

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ICC
  • Immunocytochemistry analysis of HeLa cells labeling Fas Ligand with Fas Ligand Antibody (HY-P84345) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fas Ligand Antibody (HY-P84345) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of HePG2 cells labeling Fas Ligand with Fas Ligand Antibody (HY-P84345) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Fas Ligand Antibody (HY-P84345) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:该细胞因子与 TNFRSF6/FAS 受体结合,TNFRSF6/FAS 受体可将凋亡信号传递至细胞内 (PubMed:26334989, PubMed:9228058)。它参与细胞毒性 T 细胞介导的细胞凋亡、自然杀伤细胞介导的细胞凋亡以及 T 细胞发育 (PubMed:7528780, PubMed:9228058, PubMed:9427603)。它启动抗原激活的 T 细胞中的同胞相残/自杀性激活诱导细胞死亡 (AICD),从而导致免疫应答的终止 (基于相似性)。TNFRSF6/FAS 介导的细胞凋亡在诱导外周耐受中也发挥作用 (基于相似性)。与 TNFRSF6B/DcR3 (一种阻断细胞凋亡的诱饵受体) 结合 (PubMed:27806260);诱导 FAS 介导的 NF-κB 激活,启动非凋亡信号通路 (基于相似性)。可诱导细胞凋亡,但似乎并非该过程所必需 (PubMed:27806260);胞质形式可诱导基因转录抑制。
亚细胞定位:细胞膜;单次跨膜 II 型膜蛋白;细胞质囊泡腔;溶酶体腔;分泌型;细胞核
异构体 & 翻译后修饰:P48023 有两种异构体:P48023-1:31485 Da (预测值);P48023-2:14006 Da (预测值)。
可溶性形式来源于膜结合形式,经蛋白水解加工而来。膜结合形式经历两次连续的膜内蛋白水解切割。第一次切割由 ADAM10 加工,产生一个 N 端片段,该片段缺少受体结合的胞外结构域。这种经 ADAM10 加工的 FasL (FasL APL) 残余形式仍然锚定在膜上,并进一步由 SPPL2A 加工,释放出 FasL 胞内结构域 (FasL ICD)。ADAM10 对 FasL 的释放是 T 细胞中 SPPL2A 后续进行膜内切割的先决条件;N-糖基化 (PubMed:9228058)。糖基化增强细胞凋亡活性 (PubMed:27806260);酪氨酸残基被 FGR 磷酸化;这是泛素化和后续内吞作用所必需的;单泛素化
亚基:同三聚体 (PubMed:27806260)。与 ARHGAP9、BAIAP2L1、BTK、CACNB3、CACNB4、CRK、DLG2、DNMBP、DOCK4、EPS8L3、FGR、FYB1、FYN、HCK、ITK、ITSN2、KALRN、LYN、MACC1、MIA、MPP4、MYO15A、NCF1、NCK1、NCK2、NCKIPSD 相互作用 OSTF1、PIK3R1、PSTPIP1、RIMBP3C、SAMSN1、SH3GL3、SH3PXD2B、SH3PXD2A、SH3RF2、SKAP2、SNX33、SNX9、SORBS3、SPTA1、SRC、SRGAP1、SRGAP2、SRGAP3、TEC、TJP3 和 YES1
中文名
Fas Ligand 抗体 (YA4042)
同用名
FASLG; APTL; FASL; CD95L; ALPS1B; CD95-L; TNFSF6; TNLG1A; APT1LG1; Fas Ligand Antibody
文件资料

Fas Ligand Antibody (YA4042) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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