CD42a 抗体 (YA804)

目录号: HY-P80059 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
FAQs
技术支持

CD42a Antibody (YA804) 是一个小鼠来源、无偶联标记、抗 CD42a 的 IgG1 单克隆抗体。

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规格	价格	是否有货
10 μL	￥493	In-stock
50 μL	￥1281	In-stock
100 μL	￥2100	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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验证图片
背景信息
产品资料

描述

CD42a Antibody (YA804) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD42a.

宿主

Mouse

克隆性

Monoclonal

分子量

Predicted band size: 19 kDa;

Observed band size: 28 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

P14770

基因 ID

2815 [NCBI]

免疫原

Recombinant protein within human CD42a aa 1-150/177.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:5000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:100-1:500

敏感性	Endogenous	纯度	Protein G affinity purified.
偶联	Non-conjugated	修饰	Unmodified
同型	IgG1

性状

液体

组分

Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB IHC-P

Western blot analysis of extracts from MCF-7 (lane 2(20μg) and MCF-7 (lane 3(40μg) using CD42a (HY-P80059) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD42a Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using CD42a Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景

功能：GPIb-V-IX 复合物作为 vWF 受体，介导 vWF 依赖的血小板黏附于血管。血小板黏附于动脉循环中受损的血管表面是止血的关键起始事件。GP-IX 可能有助于 GP-Ib 插入膜内并定向定位。

亚细胞定位：膜；单次跨膜 I 型膜蛋白

亚基：两个 GP-Ibβ亚基通过二硫键与一个 GP-Ibα亚基连接。GP-IX 通过非共价键与 GP-Ib 异二聚体形成复合物。

RRID

AB_3102810

反应种属数据库

Entrez Gene: 2815 Human ; 54368 Mouse

SwissProt: P14770 Human ; O88186 Mouse

OMIM: 231200 Human

研究领域

Immunology

中文名

CD42a 抗体

文件资料

Select Batch:

Data Sheet (442 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

CD42a Antibody (YA804) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

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