1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. CD79b 抗体 (YA3995)

CD79b Antibody (YA3995) 是一个小鼠来源、无偶联标记、抗 CD79b 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥609 In-stock
50 μL ¥1575 In-stock
100 μL ¥2520 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CD79b Antibody (YA3995) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD79b.

宿主

Mouse

克隆性

Monoclonal Antibody

分子量
Predicted band size: 26 kDa;
Observed band size: 30-50 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

Purified recombinant fragment of human CD79B (AA:extra 30-160) expressed in E. Coli.

应用 & 推荐
稀释比例
应用 稀释比
ICC/IF
ICC/IF: 细胞免疫荧光
1:25-1:50
FC
FC: 流式细胞术
1:200-1:400
ELISA
ELISA: 酶联免疫吸附试验
1:10000
纯度 affinity purified. 偶联 Non-conjugated
修饰 Unmodified 同型 IgG1
性状

液体

组分

Supplied in PBS with 0.05% sodium azide

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ICC
  • Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Hela cells labeling CD79b Antibody (YA3995) with CD79b Antibody (YA3995) (HY-P84298) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD79b Antibody (YA3995) ((HY-P84298) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:与 CD79A 协同作用,启动由 B 细胞抗原受体复合物 (BCR) 激活的信号转导级联反应,最终导致复合物内化、转运至晚期内体并进行抗原呈递。增强 CD79A 的磷酸化,可能是通过募集磷酸化 CD79A 的激酶,或通过募集与 CD79A 结合并保护其免于去磷酸化的蛋白质来实现的。
亚细胞定位:细胞膜;单次跨膜 I 型膜蛋白
表达水平:
组织特异性:B 细胞
异构体 & 翻译后修饰:P40259 有 3 种异构体:P40259-1:26048 Da (预测值);P40259-2:14030 Da (预测值);P40259-3:26119 Da (预测值)。
B 细胞活化后,P40259 的酪氨酸残基会被 SRC 型酪氨酸激酶 (如 BLK、LYN 和 SYK) 磷酸化。
亚基:α链和β链的异二聚体;二硫键连接。B 细胞抗原受体复合物的一部分,其中α/β链异二聚体与由两条重链和两条轻链组成的抗原特异性膜结合表面免疫球蛋白非共价结合 (PubMed:35981043)。
中文名
CD79b 抗体 (YA3995)
同用名
B29; IGB; AGM6
文件资料

CD79b Antibody (YA3995) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
CD79b Antibody (YA3995)
目录号:
HY-P84298
需求量: