2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体 流式抗体
  5. CHMP2B 抗体 (YA2855)

CHMP2B Antibody (YA2855) 是一个兔来源、无偶联标记、抗 CHMP2B 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥493 In-stock
50 μL ￥1281 In-stock
100 μL ￥2100 In-stock
250 μL   询价  

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CHMP2B Antibody (YA2855) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CHMP2B.
宿主

Rabbit
克隆性

Recombinant,Monoclonal
分子量
Predicted band size: 24 kDa;
Observed band size: 28 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human CHMP2B aa171-213.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IP
IP: 免疫沉淀
1:50
FC
FC: 流式细胞术
1:50-1:100
敏感性 Endogenous 纯度 Affinity Purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL WB ICC

  • Western blot analysis was performed on extracts from MCF-7 (lane 1, 15 μg), A549 (lane 2, 15 μg), and A431 (lane 3, 15 μg) using CHMP2B Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using CHMP2B Antibody (YA2855). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A431 cells labeling CHMP2B Antibody (HY-P83110) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHMP2B Antibody (HY-P83110) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A431 cells labeling CHMP2B Antibody (HY-P83110) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with CHMP2B Antibody (HY-P83110) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能：内体分选转运复合物 III (ESCRT-III) 的核心组分可能参与多囊泡体 (MVB) 的形成以及内体货物蛋白分选至 MVB 的过程。MVB 包含腔内囊泡 (ILV)，ILV 由内体膜内陷和裂解形成，主要被递送至溶酶体，从而降解膜蛋白，例如刺激生长因子受体、溶酶体酶和脂质。MVB 通路似乎需要 ESCRT-O、-I、-II 和 -III 复合物的顺序功能。ESCRT-III 蛋白大多在 ILV 释放之前从内陷膜上解离。ESCRT 机制也参与拓扑结构相同的膜裂变事件，例如胞质分裂的末期以及包膜病毒 (HIV-1 和其他慢病毒) 的出芽。 ESCRT-III 蛋白被认为介导必要的囊泡挤出和/或膜裂变活动，可能与 AAA ATPase VPS4 协同作用。
亚细胞定位：细胞质、胞质溶胶；晚期内体膜；外周膜蛋白
表达水平：
组织特异性：广泛表达。在脑、心脏、骨骼肌、脾脏、肾脏、肝脏、小肠、胰腺、肺、胎盘和白细胞中均有表达。在脑中，其表达于小脑、大脑皮层、延髓、脊髓、枕叶、额叶、颞叶和壳核。
异构体 & 翻译后修饰：Q9UQN3 有 2 个异构体：Q9UQN3-1：23907 Da (预测)；Q9UQN3-2：19100 Da (预测)。
亚基：可能是内体分选转运复合物 III (ESCRT-III) 的核心组分。ESCRT-III 组分被认为会多聚化，在内体周膜上形成扁平的晶格结构。ESCRT-III 可能存在多种组装形式，它们相互作用并依次发挥作用。与 CHMP2A 相互作用。与 VPS4A 相互作用。与 VPS4B 直接相互作用。
RRID
反应种属数据库

Entrez Gene: 25978 Human ; 68942 Mouse ; 363720 Rat

SwissProt: Q9UQN3 Human ; Q8BJF9 Mouse

OMIM: 609512 Human

研究领域

Signal Transduction
中文名
CHMP2B 抗体 (YA2855)
同用名
ALS17; CHMP2b; DMT1; hVps2-2; VPS2B
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

CHMP2B Antibody (YA2855) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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