2. 抗体
  3. 一抗
  4. 单克隆抗体 重组抗体
  5. Dnmt1 抗体 (YA476)

Dnmt1 Antibody (YA476) 是一个兔来源、无偶联标记、抗 Dnmt1 的 IgG 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μL ￥846 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

实现从蛋白分离到检测的高效衔接！

加购更优惠，一站式完成电泳实验！
Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Dnmt1 Antibody (YA476) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Dnmt1.
宿主

Rabbit
克隆性

Recombinant, Monoclonal
分子量
Predicted band size: 183 kDa;
Observed band size: 183 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human Dnmt1.AA range:1506-1549.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:2000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL WB ICC

  • Western blot analysis of extracts from HEK293(lane2(20μg) , HepG2(lane 3(20μg) and C6(lane 4(20ug) using Dnmt1 Antibody (HY-P80106) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling Dnmt1 with Dnmt1 Antibody (HY-P80106 )at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P80106 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of HepG2 cells labeling Dnmt1 with Dnmt1 Antibody (HY-P80106 ) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with LOX1 Antibody (HY-P80106 ) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能：甲基化 CpG 残基。优先甲基化半甲基化 DNA。在 S 期与 DNA 复制位点结合，维持新合成链的甲基化模式，这对于表观遗传至关重要。在 G2 期和 M 期与染色质结合，独立于复制维持 DNA 甲基化。它负责维持发育过程中建立的甲基化模式。DNA 甲基化与组蛋白甲基化协调一致。通过直接结合 HDAC2 介导转录抑制。与 DNMT3B 结合，并通过募集 CTCFL/BORIS，参与 BAG1 基因表达的激活，其机制是通过调节启动子组蛋白 H3 在 H3K4 和 H3K9 位点的二甲基化。可能形成一种共抑制复合物，该复合物是激活 KRAS 介导的启动子高甲基化以及结直肠癌 (CRC) 细胞中抑癌基因 (TSG) 或其他肿瘤相关基因转录沉默所必需的 (PubMed:24623306)。此外，它还对于维持未分化胚胎干细胞 (ESCs) 中基因的转录抑制状态至关重要 (PubMed:24623306)。它与肿瘤抑制基因 (TSGs) 的启动子区域结合，导致这些基因沉默 (PubMed:24623306)。它还能促进肿瘤生长 (PubMed:24623306)。
亚细胞定位：核
表达水平：
组织特异性：分布广泛；在胎儿组织、心脏、肾脏、胎盘和外周血单核细胞中高表达，在脾脏、肺、脑、小肠、结肠、肝脏和骨骼肌中表达水平较低。异构体 2 的表达量低于异构体 1。

诱导：在细胞周期的 G0 期，其丰度降低至无法检测的水平，而在进入细胞周期的 S 期后则显著增加。
亚基：同源二聚体 (PubMed:19173286)。与 E2F1、BB1 和 HDAC1 形成稳定的复合物 (PubMed:10888886)。与 DMAP1 和 HDAC2 形成复合物，并有直接相互作用 (PubMed:10888872)。
RRID
反应种属数据库

Entrez Gene: 1786 Human ; 13433 Mouse ;

SwissProt: P26358 Human ; P13864 Mouse ;

OMIM: 614116 Human

研究领域

Epigenetics and Nuclear Signaling
中文名
Dnmt1 抗体 (YA476)
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

Dnmt1 Antibody (YA476) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Dnmt1 Antibody (YA476)
目录号：
HY-P80106
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z