2. 抗体
  3. 一抗
  4. 单克隆抗体
  5. FGFR2 抗体 (YA764)

FGFR2 Antibody (YA764) 是一个小鼠来源、无偶联标记、抗 FGFR2 的 IgG1 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 μL ￥846 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

实现从蛋白分离到检测的高效衔接！

加购更优惠，一站式完成电泳实验！
Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

FGFR2 Antibody (YA764) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to FGFR2.
宿主

Mouse
克隆性

Monoclonal
分子量
Predicted band size: 92 kDa；
Observed band size: 140 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human FGFR2 / CD332.AA range:601-723.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-1:200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-1:200
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG1  
性状

液体
组分

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL WB ICC

  • Western blot analysis of extracts from HeLa(lane2(20μg) , Jurkat(lane 3(20μg) and K-562(lane 4(20ug) using FGFR2/CD332 Antibody (HY-P80398) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398)at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of SK-BR-3 cells labeling CD332 with CD332 Antibody (HY-P80398) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD332 Antibody (HY-P80398) at 1/150 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能：酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，在细胞增殖、分化、迁移和凋亡的调控以及胚胎发育的调控中发挥着至关重要的作用。它是正常胚胎模式形成、滋养层功能、肢芽发育、肺形态发生、成骨和皮肤发育所必需的。它在成骨细胞分化、增殖和凋亡的调控中起着关键作用，并且是正常骨骼发育所必需的。它促进角质形成细胞和未成熟成骨细胞的增殖，但促进分化成骨细胞的凋亡。它能磷酸化 PLCG1、FRS2 和 PAK4。配体结合可激活多个信号级联反应。PLCG1 的激活可导致细胞信号分子二酰甘油和 1,4,5-三磷酸肌醇的产生。 FRS2 的磷酸化触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集，并介导 RAS、MAPK1/ERK2、MAPK3/ERK1 和 MAP 激酶信号通路以及 AKT1 信号通路的激活。FGFR2 信号传导受泛素化、内吞和降解的抑制。导致激酶组成型激活或损害 FGFR2 正常成熟、内吞和降解的突变会导致异常信号传导。FGFR2 过表达促进 STAT1 的激活。
亚细胞定位：细胞膜；I 型单次跨膜蛋白；高尔基体；细胞质囊泡；细胞膜；I 型单次跨膜蛋白；细胞膜；I 型单次跨膜蛋白；分泌型；分泌
亚基：单体。配体结合后形成同源二聚体。主要与 FGF1 和 FGF2 相互作用，但也可与 FGF3、FGF4、FGF6、FGF7、FGF8、FGF9、FGF10、FGF17、FGF18 和 FGF22 相互作用 (体外)。配体特异性由亚型的组织特异性表达决定，第三个 Ig 样结构域的差异对配体特异性至关重要。亚型 1 对 FGF1 和 FGF2 具有高亲和力，但对 FGF7 的亲和力较低。亚型 3 对 FGF1 和 FGF7 均具有高亲和力，且对 FGF7 的亲和力远高于亚型 1 (体外)。硫酸乙酰肝素糖胺聚糖作为辅助受体可增强其对成纤维细胞生长因子 (FGFs) 的亲和力。同样，KLB 可增强其对 FGF19 和 FGF21 的亲和力。与 PLCG1、GRB2 和 PAK4 相互作用。与 FLRT2 相互作用 (基于相似性)。
RRID
反应种属数据库

Entrez Gene: 2263 Human ; 14183 Mouse ; 25022 Rat

SwissProt: P21802 Human ; P21803 Mouse ;

OMIM: 123500 Human

研究领域

Cardiovascular
中文名
FGFR2 抗体 (YA764)
文件资料

COA (241 KB)

抗体使用指南 (1083 KB)

FGFR2 Antibody (YA764) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
FGFR2 Antibody (YA764)
目录号：
HY-P80398
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z