Filamin A 抗体 (YA435)

目录号: HY-P80129 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
FAQs
技术支持

Filamin A Antibody (YA435) 是一个兔来源、无偶联标记、抗 Filamin A 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥493	In-stock
50 μL	￥1281	In-stock
100 μL	￥2100	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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验证图片
背景信息
产品资料

描述

Filamin A Antibody (YA435) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Filamin A.

宿主

Rabbit

克隆性

Recombinant,Monoclonal

分子量

Predicted band size: 281 kDa;

Observed band size: 281 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse

蛋白数据库

P21333

基因 ID

2316 [NCBI]

免疫原

Synthetic peptide corresponding to Human Filamin A.AA range:2598-2647.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500
ICC/IF ICC/IF: 细胞免疫荧光	1:50
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:100-1:400
FC FC: 流式细胞术	1:50

敏感性	Endogenous	纯度	Protein A affinity purified.
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) ,C21C2 (lane 3(20ug) and C6(lane 4(20ug) using Filamin A Antibody (HY-P80129) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of C2C12 cells labeling Filamin A with Filamin A Antibody (YA435) (HY-P80129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Filamin A Antibody (YA435) ((HY-P80129) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Filamin A with Filamin A Antibody (YA435) (HY-P80129) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Filamin A Antibody (YA435) ((HY-P80129) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景

功能：促进肌动蛋白丝的正交分支，并将肌动蛋白丝连接到膜糖蛋白上。将多种跨膜蛋白锚定到肌动蛋白细胞骨架上，并作为多种胞质信号蛋白的支架。与 FLNB 的相互作用可能允许神经母细胞从脑室区迁移到皮质板。锚定细胞表面定位的弗林蛋白酶，调节其内吞速率并指导其细胞内运输 (基于相似性)。参与纤毛发生。在血管、心脏和脑器官发育过程中，参与细胞间接触和黏附连接。通过与 SYK 相互作用，调节含 ITAM 和 ITAM 样受体的信号传导，从而在血小板形态中发挥作用，最终维持血小板细胞骨架的组织结构 (基于相似性)。在轴突导向过程中，生长锥塌陷是 SEMA3A 介导的神经元刺激所必需的 (PubMed:25358863)

亚细胞定位：细胞质，细胞皮层；细胞质，细胞骨架；胞体；细胞突起，生长锥；细胞突起，足突

表达水平：
组织特异性：普遍表达

亚基：同源二聚体。与 FOXC1 相互作用 (PubMed:15684392)。

RRID

AB_3102312

反应种属数据库

Entrez Gene: 2316 Human ; 192176 Mouse ;

SwissProt: P21333 Human ; Q8BTM8 Mouse ;

OMIM: 300049 Human

研究领域

Cell Biology

中文名

Filamin A 抗体

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

Filamin A Antibody (YA435) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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沪(浦)应急管危经许[2021]201709(QFYS)

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