Histone H3.3 抗体 (YA367)

目录号: HY-P80177 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
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Histone H3.3 Antibody (YA367) 是一个兔来源、无偶联标记、抗 Histone H3.3 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥493	In-stock
50 μL	￥1281	In-stock
100 μL	￥2100	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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验证图片
背景信息
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描述

Histone H3.3 Antibody (YA367) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Histone H3.3.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量

Predicted band size: 15 kDa;

Observed band size: 15 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse

蛋白数据库

P84243

基因 ID

3020 [NCBI]

免疫原

Synthetic peptide corresponding to Human Histone H3.3.AA range:50-100.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:500

敏感性	Endogenous	纯度	Protein A affinity purified.
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC IHC-P

Western blot analysis of extracts from Hela(lane 2(20μg) , C2C12(lane 3(20μg) and C6(lane 4(20ug) using Histone H3.3(HY-P80177 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of Hela cells labeling Histone H3.3 with Histone H3.3 Antibody (HY-P80177) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H3.3 Antibody (HY-P80177) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Histone H3.3 with Histone H3.3 Antibody (HY-P80177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Histone H3.3 Antibody (HY-P80177)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Histone H3.3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80177, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Histone H3.3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80177, 1:200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景

功能：这种变体组蛋白 H3 在活性基因的多种核小体中取代了传统的 H3。它是非分裂细胞中组蛋白 H3 的主要形式，并独立于 DNA 合成整合到染色质中。它沉积在转录基因的核小体移位位点，表明它代表了转录活性染色质的表观遗传印记。核小体包裹并压缩 DNA 形成染色质，限制了 DNA 对以 DNA 为模板的细胞机制的可及性。因此，组蛋白在转录调控、DNA 修复、DNA 复制和染色体稳定性中发挥着核心作用。DNA 可及性通过组蛋白复杂的翻译后修饰 (也称为组蛋白密码) 和核小体重塑进行调控。

亚细胞定位：细胞核；染色体

亚基：核小体是由组蛋白八聚体组成，包含两个 H2A、两个 H2B、两个 H3 和两个 H4 分子，组装成一个 H3-H4 异源四聚体和两个 H2A-H2B 异源二聚体。该八聚体包裹约 147 bp 的 DNA。核小体与 HIRA 相互作用，HIRA 是一种分子伴侣，是其整合到核小体中所必需的。核小体与 ZMYND11 相互作用；当其在赖氨酸 36 位点发生三甲基化 (H3.3K36me3) 时，核小体与 ZMYND11 相互作用。核小体存在于由 DNJC9、MCM2 和组蛋白 H3.3-H4 二聚体组成的辅助伴侣复合物中 (PubMed:33857403)。在该复合物中，核小体 (通过 C 端) 与 DNJC9 相互作用；这种相互作用是直接的 (PubMed:33857403)。与 ASF1A、MCM2、NASP 和 SPT2 相互作用 (PubMed:33857403)。与 DAXX 相互作用；该相互作用为直接相互作用 (PubMed:20504901，PubMed:34876591)。与 NASP 相互作用；NASP 是一种组蛋白伴侣，可稳定并维持可溶性组蛋白 H3-H4 二聚体 (PubMed:22195965)。

RRID

AB_3102848

反应种属数据库

Entrez Gene: 3020 Human ; 3021 Human ; 15078 Mouse ; 15081 Mouse ; 100361558 Rat 100365096

SwissProt: P84243 Human ; P84244 Mouse ; P84245 Rat

OMIM: 137800 Human

研究领域

Epigenetics and Nuclear Signaling

中文名

Histone H3.3 抗体

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

Histone H3.3 Antibody (YA367) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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