1. 抗体
  2. 一抗
  3. 单克隆抗体 流式抗体
  4. MPO 抗体 (YA3466)

MPO Antibody (YA3466) 是一个小鼠来源、无偶联标记、抗 MPO 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥493 In-stock
50 μL ¥1281 In-stock
100 μL ¥2100 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

MPO Antibody (YA3466) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MPO.

宿主

Mouse

克隆性

Monoclonal

分子量
Predicted band size: 84 kDa;
Observed band size: 84/55/37 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Recombinant protein within human Myeloperoxidase aa 550-745.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:2000-1:5000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:5000-1:10000
FC
FC: 流式细胞术
1:50-1:100
ICC/IF
ICC/IF: 细胞免疫荧光
1:100
IF-Tissue
IF-Tissue: 组织免疫荧光
1:1000-1:2000
纯度 affinity purified 偶联 Non-conjugated
修饰 Unmodified 同型 IgG1
性状

液体

组分

Supplied in 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from HL-60 (lane 2(20μg) and HL-60 (lane 3(40μg) using MPO (HY-P83736) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue using MPO Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue using MPO Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:髓过氧化物酶 (MPO) 是多形核白细胞宿主防御系统的一部分,负责对多种微生物发挥杀菌活性。在受刺激的 PMN 中,MPO 催化次卤酸 (在生理条件下主要为次氯酸) 和其他毒性中间体的生成,这些物质能显著增强 PMN 的杀菌活性 (PubMed:9922160)。MPO 还介导 α-1-微球蛋白的蛋白水解,生成反式 α-1-微球蛋白 (t-α-1-微球蛋白),后者能有效抑制低密度脂蛋白颗粒的氧化,从而减少血管损伤 (PubMed:25698971)。
亚细胞定位:溶酶体
亚基:同源二聚体;二硫键连接。每个单体由一条轻链和一条重链组成。与 CP 和 LTF 形成复合物;直接与 CP 相互作用,通过 MPO 保护 CP 的抗氧化特性 (PubMed:23843990)。
RRID
反应种属数据库
中文名
髓过氧化物酶抗体
同用名
Myeloperoxidase; MPO; 89 kDa myeloperoxidase; 84 kDa yeloperoxidase; Myeloperoxidase heavy chain; EC 1.11.1.7; PERM_HUMAN.
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
MPO Antibody (YA3466)
目录号:
HY-P83736
需求量: