2. Immunology/Inflammation Others
  3. NO Synthase Drug Derivative
  4. DHEA Benzophenone-biotin

DHEA Benzophenone-biotin 是一种具有光活性的 DHEA (HY-14650) 类似物，包含 Biotin (HY-B0511) 和 Benzophenone (HY-Y0546) 基团。DHEA Benzophenone-biotin 可抑制 DHEA 与质膜的结合，激活内皮型一氧化氮合酶 (NO synthase)。DHEA Benzophenone-biotin 经紫外线照射后可与质膜上的 DHEA 结合位点发生交联，从而鉴定相关蛋白。

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DHEA Benzophenone-biotin

DHEA Benzophenone-biotin Chemical Structure

CAS No. : 1216903-70-6

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DHEA Benzophenone-biotin 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DHEA Benzophenone-biotin is a photoactive DHEA (HY-14650) analog containing Biotin (HY-B0511) and Benzophenone (HY-Y0546) groups. DHEA Benzophenone-biotin inhibits plasma membrane binding of DHEA, activates endothelial NO synthase. DHEA Benzophenone-biotin cross-links to plasma membrane DHEA binding sites upon UV irradiation to identify associated proteins[1].

体外研究
(In Vitro)

DHEA Benzophenone-biotin (Compound DHEA–BP–Bt) (30 min) 可高亲和力结合 BAEC 细胞质膜上的 DHEA (HY-14650) 结合位点，其 Kd 值为 3.49 nM[1]。
DHEA Benzophenone-biotin (10 μM; 1 h) 可与抗生物素蛋白-琼脂糖形成复合物，该复合物对 BAEC 上的质膜 DHEA 结合位点仍保持高亲和力[1]。
DHEA Benzophenone-biotin (2 nM; 30 min) 可以一种可被 DHEA 抑制的方式特异性结合 BAEC 质膜，并且能够同时结合其质膜位点与链霉亲和素-FITC[1]。
DHEA Benzophenone-biotin (5 nM; 1 h) 经 365 nm 紫外线照射 15 分钟后，会以 62%的效率与 BAEC 细胞质膜上的 DHEA 结合位点发生不可逆交联，从而阻断后续的[3H]HDHEA 结合[1]。
DHEA Benzophenone-biotin (10 pM-100 nM; 15 min) 可以浓度依赖的方式激活 BAEC 中的 eNOS，在 10 nM 时活性达到最大增幅 81.6%，同时保留未修饰 DHEA 的生物活性[1]。
DHEA Benzophenone-biotin (1 nM) 能够以可被 DHEA 抑制的方式，特异性地与 BAEC 质膜上分子量约为 55 kDa (活细胞标记和配体印迹均可检测到) 和 80 kDa (仅活细胞标记可检测到) 的蛋白发生交联[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DHEA Benzophenone-biotin 相关抗体:

Immunofluorescence[1]

Cell Line: bovine aortic endothelial cells (BAEC)
Concentration: 2 nM;
Incubation Time: 30 min
Result: Produced cell surface fluorescence staining, which was inhibited by co-incubation with DHEA.
分子量

1022.34

Formula

C57H79N7O8S
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

DHEA Benzophenone-biotin 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DHEA Benzophenone-biotin1216903-70-6NO SynthaseDrug DerivativeNitric oxide synthasesNOSDrug derivativeconfocal microscopyeNOSplasma membrane DHEA receptorphotoaffinity cross-linking agentstreptavidin-FITCplasma membrane DHEA binding sitesBAECendothelial nitric oxide synthaseavidin-agarosenitrocellulose-bound proteinsInhibitorinhibitorinhibit

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