2. Protein Tyrosine Kinase/RTK
  3. FLT3
  4. FLT3-IN-40

FLT3-IN-40 (compound 1) 是一种 I 型 ATP 竞争性 FLT3 抑制剂，其 IC50 为 16.26 nM。FLT3-IN-40 可降低 FLT3 的自身磷酸化水平及下游 ERK 的磷酸化水平。FLT3-IN-40 具有抗增殖、细胞周期调节和诱导凋亡的活性。FLT3-IN-40 可用于急性髓系白血病的相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

我们将采用定制合成服务的方式为您快速提供所需产品和技术服务

FLT3-IN-40

FLT3-IN-40 Chemical Structure

CAS No. : 3075541-07-7

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

FLT3-IN-40 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

FLT3-IN-40 (compound 1) is a type I ATP-competitive FLT3 inhibitor with an IC50 of 16.26 nM. FLT3-IN-40 reduces the autophosphorylation level of FLT3 and the phosphorylation level of downstream ERK. FLT3-IN-40 exhibits antiproliferative, cell cycle regulatory and apoptosis-inducing activities. FLT3-IN-40 can be used in research related to acute myeloid leukemia[1].

体外研究
(In Vitro)

FLT3-IN-40 (compound 1) (0.00001-100 nM; sufficient to measure antiproliferative effects) 可强效抑制 MV4-11 细胞增殖，其 IC50 为 0.0021 nM，呈现出渐进的双相剂量反应特征[1]。
FLT3-IN-40 (0.001-10 nM) 是一种 ATP 竞争性 I 型 FLT3 抑制剂，可抑制纯化的 FLT3 激酶，其 IC50 为 16.26 nM；在抑制 p-ERK 时，对 MV4-11 细胞中 FLT3 通路信号传导的 IC50 为 0.80 nM[1]。
FLT3-IN-40 (足以检测抗增殖效应) 可抑制 Molm14 细胞增殖，其 IC50 为 1.42 nM；而对缺乏 FLT3 激活突变的 K562 细胞 (IC50 1583 nM) 和 Ba/F3 细胞 (IC50 8467 nM)，该化合物的抗增殖活性则弱得多[1]。
FLT3-IN-40 (1 pM-10 nM; 72 h) 在皮摩尔浓度下对 MV4-11 细胞仅诱导极少量细胞凋亡，但在 10 nM 处理 72 h 后可显著触发早期 (17.3%) 和晚期 (10.3%) 细胞凋亡[1]。
FLT3-IN-40 (1 pM-10 nM; 72 h) 会在皮摩尔浓度下诱导 MV4-11 细胞出现中度 G1 期阻滞稳定，并在 10 nM 处理 72 h 后诱导强烈的 G1 期细胞周期阻滞[1]。
FLT3-IN-40 (1 nM; 10 nM) 具有高激酶选择性，在 1 nM 和 10 nM 浓度下测试的 96 种激酶中，仅与 FLT3 发生强结合[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

FLT3-IN-40 相关抗体:

Cell Proliferation Assay[1]

Cell Line: MV4-11 (FLT3-ITD positive acute myeloid leukemia) cells
Concentration: 0.00001-100 nM
Incubation Time: sufficient to measure antiproliferative effects
Result: Inhibited MV4-11 cell proliferation with an IC50 of 0.0021 nM (or 2.13 pM).
Achieved ~50% inhibition at 2 pM and >90% inhibition at 20 nM, with a gradual, biphasic dose-response curve.

Apoptosis Analysis[1]

Cell Line: MV4-11 cells
Concentration: 1 pM-10 nM
Incubation Time: 72 h
Result: Induced early apoptotic cell percentages of 2.1%, 2.3%, 3.4%, and 4.8% at 1 pM, 10 pM, 100 pM, and 1 nM respectively, with minimal late apoptosis.
Triggered early apoptotic cell percentage of 17.3% and late apoptotic cell percentage of 10.3% at 10 nM, showing a marked increase in apoptosis at nanomolar concentrations.

Cell Cycle Analysis[1]

Cell Line: MV4-11 cells
Concentration: 1 pM-10 nM
Incubation Time: 72 h
Result: Increased G1 phase cell percentages to 52.4%, 53.5%, and 54.5% at 1 pM, 10 pM, and 100 pM respectively (compared to 47.2% for DMSO control).
Increased G1 phase percentage to 58.1% at 1 nM, and to 68.7% at 10 nM with a corresponding decrease in G2 phase from 8.93% at 1 nM to 3.01% at 10 nM.
分子量

426.47

Formula

C24H22N6O2
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

FLT3-IN-40 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FLT3-IN-403075541-07-7FLT3Cluster of differentiation antigen 135CD135Fms like tyrosine kinase 3ERK phosphorylationK562 cellMolm14 cellautophosphorylationacute myeloid leukemiacell cycle arrestapoptosisMV4-11 cellBa/F3 cellInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
FLT3-IN-40
目录号：
HY-182004
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z