2. Immunology/Inflammation
  3. CD1
  4. GCB-27a

GCB-27a 是一种 CD1d 结合型免疫刺激剂和抗肿瘤剂。GCB-27a 可与 CD1d 结合形成稳定复合物并呈递给 NKT 细胞，通过支链构象限制增强 CD1d A'口袋内的疏水相互作用。GCB-27a 可诱导偏向 Th1 型的免疫应答，驱动 IFN−γ 产生且 IL-4 水平受限。GCB-27a 可用于黑色素瘤肺转移的相关研究。

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GCB-27a

GCB-27a Chemical Structure

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GCB-27a 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GCB-27a is a CD1d-binding immunostimulant and antitumor agent. GCB-27a binds to CD1d to form a stable complex and presents it to NKT cells, enhancing hydrophobic interactions within the A' pocket of CD1d through branched-chain conformation restriction. GCB-27a induces a Th1-biased immune response, drives IFN−γ production and limits IL-4 levels. GCB-27a is applicable to research related to melanoma lung metastasis[1].

体外研究
(In Vitro)

GCB-27a (1-1000 nM; 72 h) 可在体外强效刺激 C57BL/6 小鼠脾细胞中偏向 Th1 型的细胞因子产生，在所有测试浓度下 IFN-γ 分泌水平均升高，且无相关细胞毒性[1]。
GCB-27a (100 nM; 2-24 h) 与小鼠 CD1d 在 BMDC 表面形成的复合物比 αGalCer 更稳定、持续时间更长，这一点可通过 L363 抗体结合作用的增强和延长得到证实[1]。
GCB-27a (24 h) 可在人 NKT 细胞中强效诱导 Th1 偏向型细胞因子的产生，其 IFN-γ+细胞的频率较 αGalCer 高 2 倍，且对 IL-4 的诱导效果相当[1]。
GCB-27a 对人源和鼠源 CD1d 的结合亲和力均强于 αGalCer，这是由于其在 CD1d A' 口袋内的疏水相互作用和构象预组织得到了优化[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GCB-27a 相关抗体:
体内研究
(In Vivo)

GCB-27a (2.33 nmol per mouse; i.p.; single injection) 可在 BALB/c 小鼠中诱导强效的 Th1 偏向型免疫应答，其 IFN-γ 水平较 αGalCer 高 10.9 倍，同时维持相当的 IL-4 水平[1]。
GCB-27a (2.33 nmol per mouse; i.p.; single injection) 可强效激活 C57BL/6 小鼠体内的固有免疫细胞，增强树突状细胞 (DC) 成熟标志物的表达，并提高分泌 IFN-γ 的 NK 细胞的频率[1]。
GCB-27a (0.58 nmol per mouse; i.p.; single injection) 在小鼠黑色素瘤肺转移模型中展现出强效抗肿瘤活性，通过持续的 Th1 偏向型免疫应答，相较于 αGalCer 使转移结节数减少 89%[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice (n=5)[1]
Dosage: 2.33 nmol per mouse
Administration: i.p.; single injection
Result: Induced a 10.9-fold increase in serum IFN-γ levels relative to αGalCer at 24 hours post-injection.
Maintained IL-4 levels comparable to those induced by αGalCer.
Demonstrated the strongest Th1 selectivity among all tested analogs, with the highest IFN-γ/IL-4 ratio fold change over αGalCer.
Confirmed sustained Th1-biased activity via time-dependent cytokine analysis, with peak IFN-γ levels observed at 24 hours post-injection.
Animal Model: C57BL/6 mice (n=3)[1]
Dosage: 2.33 nmol per mouse
Administration: i.p.; single injection
Result: Markedly increased CD80 and CD86 expression on CD11c+ DCs relative to αGalCer.
Elevated the frequency of IFN-γ-producing NK cells to ~20% of splenic NK cells, a significant increase compared to αGalCer.
Animal Model: C57BL/6 mice (n=6; melanoma lung metastasis model)[1]
Dosage: 0.58 nmol per mouse
Administration: i.p.; single injection
Result: Elicited significantly elevated serum IFN-γ levels relative to αGalCer, while IL-4 levels remained comparable to αGalCer.
Achieved an 89% decrease in lung metastatic nodule counts relative to the αGalCer-treated group.
Revealed near-complete inhibition of lung metastasis via histological analysis.
分子量

894.31

Formula

C52H95NO10
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GCB-27a 相关分类

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  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GCB-27aCD1dendritic cellsCD1dNKT cellsIFN-γIL-4mouse splenocytesnatural killer cellsC57BL/6 miceBALB/c micemouse melanoma lung metastasis modelInhibitorinhibitorinhibit

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