2. Immunology/Inflammation
  3. CD1
  4. GCB-27b

GCB-27b 是一种可结合 CD1d 的免疫刺激剂。GCB-27b 可与 CD1d 形成稳定且长效的复合物，该复合物会呈递给 NKT 细胞的 TCR 以驱动免疫应答。GCB-27b 可在 *Mus musculus*(小家鼠) 中诱导 Th1 偏向型免疫应答，导致 IFN−γ 的高表达，同时 IL-4 水平受到限制。GCB-27b 适用于黑色素瘤肺转移相关研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

GCB-27b

GCB-27b Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

GCB-27b 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

GCB-27b is an immunostimulant that binds to CD1d. GCB-27b forms a stable and long-lasting complex with CD1d, which is presented to the TCR of NKT cells to drive immune responses. GCB-27b induces a Th1-skewed immune response in *Mus musculus*, resulting in high expression of IFN−γ with restricted IL-4 levels. GCB-27b is applicable to research related to lung metastasis of melanoma[1].

体外研究
(In Vitro)

GCB-27b (1-1000 nM; 72 h) 可在体外强效刺激 C57BL/6 小鼠脾细胞中偏向 Th1 型的细胞因子产生，且未观察到细胞毒性[1]。
GCB-27b (100 nM; 2-24 h) 可在体外与小鼠骨髓树突状细胞 (BMDC) 表面的 CD1d 形成更稳定且持久的复合物，在孵育后 12、18 和 24 h 时的表面呈递水平相较于 αGalCer 有所增强[1]。
GCB-27b (24 h) 可在体外强效诱导人 PBMC 来源 NKT 细胞向 Th1 型偏向的活化，其 IFN-γ+细胞的频率较 αGalCer 高 2 倍[1]。
GCB-27b 对人类和小鼠 CD1d 的计算结合亲和力均高于 αGalCer，二者的对接评分均为 20，这是由于其在 CD1d A' 口袋中形成了经优化的疏水相互作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

GCB-27b 相关抗体:
体内研究
(In Vivo)

GCB-27b (2.33 nmol per mouse; i.p.; single dose) 在 BALB/c 小鼠中诱导的血清 IFN-γ 水平较 αGalCer 高 10.5 倍，且 IL-4 水平与 αGalCer 相近，显示出强效的 Th1 偏向型免疫活性[1]。
GCB-27b (2.33 nmol per mouse; i.p.; single dose) 可在 C57BL/6 小鼠中强效激活脾脏树突状细胞 (通过上调 CD80 和 CD86 的表达) 和自然杀伤 (NK) 细胞 (通过促进 IFN−γ 的产生)，显示出强大的固有免疫刺激活性[1]。
GCB-27b (0.58 nmol per mouse; i.p.; single dose) 在 C57BL/6 小鼠中可使 B16−F10 黑色素瘤肺转移结节数较 αGalCer 降低 88%，该作用由强效的 Th1 偏向性细胞因子应答驱动[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (n=5)[1]
Dosage: 2.33 nmol per mouse
Administration: i.p.; single dose
Result: Induced a 10.5-fold increase in serum IFN-γ levels (measured at 24 h postinjection) relative to αGalCer.
Showed IL-4 levels (measured at 2 h postinjection) comparable to those induced by αGalCer.
Demonstrated IL-4 levels peaked at 2 h then declined, while IFN-γ levels peaked at 24 h then declined, consistent with strong Th1-biased activity.
Animal Model: C57BL/6 (n=3)[1]
Dosage: 2.33 nmol per mouse
Administration: i.p.; single dose
Result: Induced significantly increased mean fluorescence intensity (MFI) of CD80 and CD86 on CD11c+ dendritic cells (DCs) relative to αGalCer.
Elevated the proportion of IFN-γ+ NK cells in the spleen to 17.6%, which is significantly higher than the proportion induced by αGalCer.
Animal Model: C57BL/6 (n=6; melanoma lung metastasis model)[1]
Dosage: 0.58 nmol per mouse
Administration: i.p.; single dose
Result: Induced significantly elevated serum IFN-γ levels (measured at 24 h postinjection) relative to αGalCer, with IL-4 levels (measured at 2 h postinjection) comparable to those induced by αGalCer.
Reduced lung metastatic nodule counts by 88% relative to the αGalCer-treated group, with near-complete inhibition of lung metastasis confirmed by H&E staining.
分子量

894.31

Formula

C52H95NO10
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

GCB-27b 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GCB-27bCD1NKT cellsIFN-γhuman PBMC-derived NKT cellsC57BL/6 mouse splenocytesmelanoma lung metastasisBALB/c miceNKT cell TCRCD1dmouse BMDCsIL-4Inhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
GCB-27b
目录号：
HY-181740
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z