2. Academic Validation
  3. Microarray based screening of peptide nano probes for HER2 positive tumor

Microarray based screening of peptide nano probes for HER2 positive tumor

  • Anal Chem. 2015 Aug 18;87(16):8367-72. doi: 10.1021/acs.analchem.5b01588.
Zihua Wang,  Weizhi Wang,  Xiangli Bu,  Zewen Wei,  Lingling Geng,  Yue Wu 1,  Chengyan Dong 1,  Liqiang Li 1,  Di Zhang,  Shu Yang,  Fan Wang 1,  Christopher Lausted 2,  Leroy Hood 2,  Zhiyuan Hu 2 3
Affiliations

Affiliations

  • 1 ‡Medical Isotopes Research Center, Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing China, 100191.
  • 2 §Institute for Systems Biology, 401 Terry Avenue North, Seattle, Washington 98109, United States.
  • 3 ∥Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing China, 102206.
PMID: 26218790 DOI: 10.1021/acs.analchem.5b01588
Abstract

Peptides are excellent biointerface molecules and diagnostic probes with many advantages such as good penetration, short turnover time, and low cost. We report here an efficient peptide screening strategy based on in situ single bead Sequencing on a microarray. Two novel peptides YLFFVFER (H6) and KLRLEWNR (H10) specifically binding to the tumor biomarker human epidermal growth factor receptor 2 (HER2) with aKD of 10(-8) M were obtained from a 10(5) library. Conjugated to nanoparticles, both the H6 and H10 probes showed specific accumulation in HER2-positive tumor tissues in xenografted mice by in vivo imaging.

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