2. Academic Validation
  3. Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule

Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule

  • ACS Chem Biol. 2017 Oct 20;12(10):2546-2551. doi: 10.1021/acschembio.7b00647.
Hidefumi Iwashita 1 2,  Satoru Torii 3,  Noriyoshi Nagahora 2,  Munetaka Ishiyama 1,  Kosei Shioji 2,  Kazumi Sasamoto 1,  Shigeomi Shimizu 3,  Kentaro Okuma 2
Affiliations

Affiliations

  • 1 Dojindo Laboratories , Tabaru 2025-5, Mashiki-machi, Kumamoto 861-2202, Japan.
  • 2 Department of Chemistry, Faculty of Science, Fukuoka University , Jonan-Ku, Fukuoka 814-0180, Japan.
  • 3 Department of Pathological Cell Biology, Medical Research Institute, Tokyo Medical and Dental University , 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
PMID: 28925688 DOI: 10.1021/acschembio.7b00647
Abstract

There has been a growing interest in Mitophagy, mitochondria-selective Autophagy, which plays an essential role in maintaining intracellular homeostasis. We have developed a small-molecule fluorescent probe, Mtphagy Dye, for visualizing Mitophagy, which was readily synthesized from a known perylene derivative, perylene-3,4-dicarboxylic anhydride. Mtphagy Dye has suitable fluorescent properties for detecting mitochondrial acidification during Mitophagy in the long-wavelength region that does not damage mitochondria. Using Mtphagy Dye, we were able to visualize Mitophagy with both cases of Parkin-dependent and -independent HeLa cells.

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