2. Cell Cycle/DNA Damage
  3. ClpP
  4. WY165

WY165 是一种双功能分子，由 TR79 (线粒体蛋白酶复合物酪蛋白水解酶 P (ClpP) 的激活剂) 与脱硫生物素连接而成。WY165 可选择性降解定位于线粒体基质的单体链霉亲和素 (mSA) 及其融合蛋白，其 DC50 为 197 nM。WY165 通过降低过表达 mSA-STMP1 细胞中 mSA 与短跨膜蛋白 1 (STMP1) 融合蛋白 (mSA-STMP1) 的水平，从而恢复线粒体形态。WY165 可用于癌症、神经退行性疾病、心血管疾病和代谢性疾病的研究。

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WY165

WY165 Chemical Structure

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WY165 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

WY165 is a bifunctional molecule comprising TR79, an activator of the mitochondrial protease complex caseinolytic protease P (ClpP), linked to desthiobiotin. WY165 mediates selective degradation of monomeric streptavidin (mSA) and its fusion proteins localized to the mitochondrial matrix with a DC50 of 197 nM. WY165 restores mitochondrial morphology by reducing the level of mSA fused to short transmembrane protein 1 (mSA-STMP1) in cells overexpressing mSA-STMP1. WY165 can be used for research in cancer, neurodegenerative diseases, cardiovascular diseases, and metabolic diseases[1].

IC50 & Target

caseinolytic protease P (ClpP), monomeric streptavidin (mSA)
体外研究
(In Vitro)

WY165 (10-10000 nM；24 小时) 以剂量依赖的方式降低 mSA 水平[1]。
WY165 (1 μM；24 小时) 对 mSA 的降解活性可被过量的脱硫生物素 (1 mM) 抑制，表明 [mSA-WY165-ClpP] 三元复合物的形成是必需的[1]。
WY165 (0.3-30 μM；2-12 小时) 在瞬时表达线粒体 cox8-mSA-FLAG 的 HeLa 和 MCF7 细胞中以剂量和时间依赖的方式降低线粒体中的 mSA 水平，对 mSA 的 DC50 值分别为 4.8 μM 和 0.96 μM[1]。 WY165 (10 μM；48 h) 在 HeLa 细胞中诱导的 mSA 降解依赖于 ClpP[1]。
WY165 (1-30 μM) 在瞬时表达 cox8-His6-mSA-STMP1-FLAG 的 HeLa 细胞中以剂量依赖的方式降低 mSA-STMP1 的表达[1]。
WY165（1 μM）可在 HeLa 细胞中恢复 mSA-STMP1 导致的线粒体形态异常[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

WY165 相关抗体:

Western Blot Analysis[1]

Cell Line: HeLa cells transiently expressing mitochondrial cox8-mSA-FLAG
Concentration: 1 μM; 3 μM; 10 μM; 30 μM
Incubation Time: 12 h
Result: Successfully reduced the mSA level in the concentration of 10 μM and 30 μM.

Western Blot Analysis[1]

Cell Line: HeLa cells transiently expressing mitochondrial cox8-mSA-FLAG
Concentration: 10 μM
Incubation Time: 2 h; 4 h; 6 h; 12 h
Result: Successfully reduced the mSA level upon 4 h treatment.

Western Blot Analysis[1]

Cell Line: MCF7 cells transiently expressing mitochondrial cox8-mSA-FLAG
Concentration: 0.3 μM; 1 μM; 3 μM; 10 μM; 30 μM
Incubation Time: 12 h
Result: Significantly reduced the mSA level when concentration over 1 μM.

Western Blot Analysis[1]

Cell Line: MCF7 cells transiently expressing mitochondrial cox8-mSA-FLAG
Concentration: 3 μM
Incubation Time: 2 h; 4 h; 6 h; 12 h
Result: Significantly reduced the mSA level upon 2 h treatment.

Western Blot Analysis[1]

Cell Line: HeLa cells with ClpP knockdown by transfection of siClpP
Concentration: 10 μM
Incubation Time: 48 h
Result: Failed to degrade mSA in the presence of ClpP RNAi.

Western Blot Analysis[1]

Cell Line: HeLa cells transiently expressing cox8-His6-mSA-STMP1-FLAG
Concentration: 1 μM; 3 μM; 10 μM; 30 μM
Incubation Time: 48 h
Result: Decreased mSA-STMP1 in a dose-dependent manner.
分子量

927.02

Formula

C46H61F3N8O9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

WY165 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WY165WY 165WY-165ClpPCaseinolytic protease PTR79caseinolytic protease Pdesthiobiotinmonomeric Streptavidinmitochondrial morphologyshort transmembrane protein 1cancerneurodegenerative diseasescardiovascular diseasesmetabolic diseasesInhibitorinhibitorinhibit

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