MES 一水合物

别名 4-吗啉乙磺酸 一水合物, 2-(N-吗啉基)乙磺酸
英文别名 MES monohydrate|145224-94-8|2-Morpholinoethanesulfonic acid hydrate|MES hydrate|1266615-59-1|2-morpholin-4-ylethanesulfonic acid;hydrate|MFCD00149409|2-Morpholinoethanesulfonicacidxhydrate|4-Morpholineethanesulfonic acid monohydrate|2-Morpholinoethanesulf
规格或纯度 超纯生物试剂级，用于分子生物学，≥99.5%（T）
英文名称 MES monohydrate
产品介绍 一般描述 MES是为生物学应用开发的所谓的“良好”缓冲液之一。不建议在pH 7.4下使用MES；可考虑其他缓冲液。所有质量的痕量离子已经过测试，并且所有离子均可用于生物和生物化学应用。该产品可用于分子生物学。 应用 MES一水合物用于： 制备用于悬浮和稀释原生质体的溶液，从而使用血细胞计数器对原生质体的密度和数量进行测定 作为Murashige和Skoog基础盐培养基的组成部分 制备总离子强度调节缓冲液（TISAB） 其他说明 溶解度/稳定性： MES可溶于水，浓度为0.5 M或更高时，为澄清无色溶液。溶液的pH值应在2.5到5之间，具体取决于浓度。在2-8℃ 下，溶液可稳定保存数月。 灭菌： 灭菌应通过0.2 uM过滤器进行过滤。不建议将任何磺酸缓冲液进行高压灭菌。如果缓冲液必须不含核酸酶，则最好对水进行处理，然后在高压灭菌后添加缓冲液固体。将MES溶液高压灭菌后，其会变成黄色（尽管pH值不会发生太大变化）。黄色分解产物的成分仍未知。 制备说明 可以通过用***滴定游离酸至所需的pH（pKa =±1）来制备含MES游离酸的缓冲液。或者，可以将一定体积的MES游离酸和MES钠等摩尔溶液混合以达到所需的pH值。使用储备溶液制备给定pH值缓冲液的标准混合表已有报道。 General Description MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in molecular biology. Application MES Monohydrate has been used: In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometer As a component of Murashige and Skoog basal salt media In the preparation of total ionic strength adjustment buffer (TISAB) Other Notes Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months. Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.