1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. CD14 抗体

CD14 Antibody 是一个兔来源、无偶联标记、抗 CD14IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥504 In-stock
50 μL ¥1312 In-stock
100 μL ¥2100 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CD14 Antibody is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD14.

宿主

Rabbit

克隆性

Monoclonal,Recombinant

分子量

Predicted band size: 40kDa; Observed band size: 55kDa

反应种属
Human
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human CD14 aa300-340.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL IHC IHC-P
  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using CD14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81096, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using CD14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81096, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using CD14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81096, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using CD14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81096, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using CD14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81096, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using CD14 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81096, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using CD14 Antibody (HY-P81096, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue using CD14 Antibody (HY-P81096, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using CD14 Antibody (HY-P81096, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue using CD14 Antibody (HY-P81096, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using CD14 Antibody (HY-P81096, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using CD14 Antibody (HY-P81096, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:细菌脂多糖的辅助受体 (PubMed:1698311, PubMed:23264655)。与脂多糖结合蛋白 (LBP) 协同作用,结合单体脂多糖并将其递送至 LY96/TLR4 复合物,从而介导针对细菌脂多糖 (LPS) 的先天免疫反应 (PubMed:20133493, PubMed:22265692, PubMed:23264655)。通过 MyD88、TIRAP 和 TRAF6 发挥作用,导致 NF-κB 激活、细胞因子分泌和炎症反应 (PubMed:8612135)。该簇作为 TLR2:TLR6 异二聚体在二酰化脂肽刺激下的辅助受体,以及 TLR2:TLR1 异二聚体在三酰化脂肽刺激下的辅助受体,触发细胞表面信号传导,随后通过脂筏依赖性途径靶向高尔基体 (PubMed:16880211)。该簇可结合带负电荷的低密度脂蛋白 (LDL (-)),并介导 LDL (-) 诱导的细胞因子释放 (PubMed:23880187)。
亚细胞定位:细胞膜;脂锚;GPI 锚;分泌型;膜筏;高尔基体
表达水平:
组织特异性:在巨噬细胞上检测到 (蛋白水平) (PubMed:1698311) 。在单核细胞表面表达强烈,在粒细胞表面表达较弱;也在大多数组织巨噬细胞中表达。

诱导:27-羟基胆固醇可显著诱导单核细胞中CD14的表达,激活单核细胞/巨噬细胞,从而加速LPS介导的炎症反应。同时,可溶性CD14的分泌也增强。
亚基:与 LPS 结合的 LPB 相互作用 (PubMed:1698311,PubMed:23264655)。属于脂多糖 (LPS) 受体,一种多蛋白复合物,至少包含 CD14、LY96 和 TLR4 (PubMed:11274165)。
RRID
反应种属数据库

Entrez Gene: 929 Human

SwissProt: P08571 Human

OMIM: 158120 Human

中文名
内毒素受体抗体
同用名
CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_MOUSE; Myeloid cell-specific leucine-rich glycoprotein.
文件资料

CD14 Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD14 Antibody
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