1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. CD63 抗体 (YA5644)

CD63 Antibody (YA5644) 是一个小鼠来源、无偶联标记、抗 CD63 的 IgG2a 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥525 In-stock
50 μL ¥1365 In-stock
100 μL ¥2205 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CD63 Antibody (YA5644) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD63.

宿主

Mouse

克隆性

Monoclonal

分子量
Predicted band size: 26 kDa;
Observed band size: 30-65 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

Synthesized peptide derived from human CD63 AA range: 100-200

应用 & 推荐
稀释比例
应用 稀释比
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:200-1000
WB
WB: 蛋白质免疫印迹
1:500-2000
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-500
ELISA
ELISA: 酶联免疫吸附试验
1:1000-5000
纯度 Protein G 偶联 Non-conjugated
修饰 Unmodified 同型 IgG2a
性状

液体

组分

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ICC
  • Immunocytochemistry analysis of A431 cells labeling CD63 with CD63 antibody (HY-P85952) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD63 antibody (HY-P85952) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A431 cells labeling CD63 with CD63 antibody (HY-P85952) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CD63 antibody (HY-P85952) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:作为 TIMP1 的细胞表面受体,它参与细胞信号级联的激活。它参与 ITGB1 和整合素信号的激活,进而激活 AKT、FAK/PTK2 和 MAP 激酶。它通过激活 AKT 和 FAK/PTK2,促进细胞存活、肌动蛋白细胞骨架重组、细胞黏附、铺展和迁移。它通过调节 KDR/VEGFR2 的内吞作用,参与 VEGFA 信号通路。它参与细胞内囊泡运输过程,并且是 PMEL 腔内结构域正常运输所必需的,而 PMEL 腔内结构域对于黑素细胞的发育和成熟至关重要。它通过调节 SELP 的运输,参与白细胞与内皮细胞的黏附。可能在肥大细胞响应 Ms4a2/FceRI 刺激而脱颗粒的过程中发挥作用,但在响应其他刺激而脱颗粒的过程中则不发挥作用。
亚细胞定位:细胞膜;多通道膜蛋白;溶酶体膜;多通道膜蛋白;晚期内体膜;多通道膜蛋白;内体,多泡体;黑素体;分泌型胞外体;细胞表面
表达水平:
组织特异性:在血小板 (蛋白质水平) 中检测到。发育不良痣、放射状生长期原发性黑色素瘤、造血细胞、组织巨噬细胞。
异构体 & 翻译后修饰:P08962 有 3 种异构体:P08962-1:25637 Da (预测值);P08962-2:23430 Da (预测值);P08962-3:17271 Da (预测值)。
在未受刺激的血小板中,P08962 的棕榈酰化水平较低。当血小板被凝血酶激活时 (体外),其棕榈酰化水平升高。
亚基:与 TIMP1 和 ITGB1 相互作用,并将 TIMP1 募集到 ITGB1 (PubMed:16917503, PubMed:24635319)。与 CD9 相互作用。在与 CD9 和 ITGB3 形成的复合物中被发现 (PubMed:19640571)。与 PMEL 相互作用 (PubMed:21962903)。与 KDR/VEGFR2 相互作用;在与 ITGB1 和 KDR/VEGFR2 形成的复合物中被发现,并且是募集 KDR 到 ITGB1 复合物所必需的 (PubMed:23632027)。与 SYT7 相互作用 (基于相似性)。
RRID
中文名
CD63 抗体 (YA5644)
文件资料

CD63 Antibody (YA5644) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
CD63 Antibody (YA5644)
目录号:
HY-P85952
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