1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体 流式抗体
  4. CDC45 抗体 (YA3158)

CDC45 Antibody (YA3158) 是一个兔来源、无偶联标记、抗 CDC45 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥493 In-stock
50 μL ¥1281 In-stock
100 μL ¥2100 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

CDC45 Antibody (YA3158) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC45.

宿主

Rabbit

克隆性

Recombinant,Monoclonal

分子量
Predicted band size: 66 kDa;
Observed band size: 66 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human CDC45 aa500-566/566.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:1000-1:2000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-1:200
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
FC
FC: 流式细胞术
1:50-1:100
敏感性 Endogenous 纯度 Affinity Chromatography
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
WB
  • Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), K562 (lane 2, 15 μg), NCI-H1299 (lane 3, 15 μg), and Hela (lane 4, 15 μg) using CDC45 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), MCF-7 (lane 3), 293T (lane 4), HT-1080 (lane 5), A549 (lane 6), and HepG2 (lane 7, Negative), using CDC45 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

背景
功能:启动染色体 DNA 复制所必需。它是 CDC45-MCM-GINS (CMG) 解旋酶的核心组成部分,该解旋酶是一种分子机器,在复制过程中解开模板 DNA,复制体正是围绕它构建的。
亚细胞定位:细胞核;染色体
表达水平:
组织特异性:该基因广泛表达,在人睾丸和胸腺以及胎儿肝脏中含量最高。
异构体 & 翻译后修饰:O75419 有 3 个异构体:O75419-1:65569 Da (预测);O75419-2:60245 Da (预测);O75419-3:68768 Da (预测)。
亚基:CMG 解旋酶复合物的组成部分,该复合物是由 CDC45 和四聚体 GINS 复合物稳定的六聚体 MCM2-7 亚基环状结构 (PubMed:32453425, PubMed:34694004, PubMed:34700328)。与 ORC2 相关。与 HELB 相互作用 (PubMed:25933514)。
反应种属数据库
研究领域

Epigenetics and Nuclear Signaling

中文名
CDC45 抗体 (YA3158)
同用名
CDC45L; CDC45L2; PORC-PI-1; CDC45
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
CDC45 Antibody (YA3158)
目录号:
HY-P83413
需求量: