1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. FOXM1 抗体 (YA1112)

FOXM1 Antibody (YA1112) 是一个兔来源、无偶联标记、抗 FOXM1 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥504 In-stock
50 μL ¥1312 In-stock
100 μL ¥2100 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

FOXM1 Antibody (YA1112) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FOXM1.

宿主

Rabbit

克隆性

Recombinant,Monoclonal

分子量
Predicted band size: 84 kDa;
Observed band size: 110 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Recombinant protein within human FOXM1 aa 1-400.

应用 & 推荐
稀释比例
应用 稀释比
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:1000
ICC/IF
ICC/IF: 细胞免疫荧光
1:100
敏感性 Endogenous 纯度 Affinity Purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ICC
  • Immunocytochemistry analysis of Hela cells labeling FOXM1 Antibody (HY-P80467) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXM1 Antibody (HY-P80467) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of Hela cells labeling FOXM1 Antibody (HY-P80467) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with FOXM1 Antibody (HY-P80467) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:转录因子,调控细胞周期基因的表达,这些基因对 DNA 复制和有丝分裂至关重要 (PubMed:19160488, PubMed:20360045)。在细胞增殖调控中发挥作用 (PubMed:19160488)。也参与 DNA 断裂修复,是 DNA 损伤检查点反应的一部分 (PubMed:17101782)。促进 PHB2 的转录 (PubMed:33754036)。
亚细胞定位:
表达水平:
组织特异性:该基因在胸腺、睾丸、小肠、结肠以及卵巢中表达。似乎仅在含有增殖/周期细胞的成年器官中表达,或在生长因子刺激下表达。也表达于肿瘤来源的上皮细胞系中。在静止细胞中不表达。异构体 2 在睾丸中高表达。

诱导:在肝脏再生和氧化应激过程中诱导产生

阳性样本:Human colon tissue, human colon cancer tissue, HeLa, MEF.
异构体 & 翻译后修饰:Q08050 有 3 种异构体:Q08050-1:84283 Da (预测值);Q08050-2:82685 Da (预测值);Q08050-3:88596 Da (预测值)。
在 M 期 (有丝分裂期) 发生磷酸化 (PubMed:17101782, PubMed:19160488, PubMed:30139873)。DNA 损伤后,检查点激酶 CHEK2 介导的磷酸化可提高 FOXM1 蛋白的稳定性,这可能刺激参与 DNA 修复的基因转录 (PubMed:17101782)。在 S 期晚期和 G2 期,CDK1 磷酸化该蛋白,形成 PLK1 POLO 盒结构域的结合位点 (PubMed:19160488, PubMed:30139873)。随后,PLK1 结合并磷酸化 FOXM1,激活转录活性,并增强关键有丝分裂调节因子的表达 (PubMed:19160488)。此外,该蛋白还可被 GSK3B 磷酸化,导致泛素化和蛋白酶体降解 (PubMed:26912724);或以 FBXW7 依赖的方式泛素化,最终导致蛋白酶体降解。
亚基:与由长链非编码 RNA LINC-PINT 环状形式编码的 PINT87aa 相互作用;该相互作用抑制 FOXM1 介导的 PHB2 转录。
RRID
反应种属数据库
研究领域

Epigenetics and Nuclear Signaling

中文名
FOXM1 抗体 (YA1112)
同用名
FOXM1; FKHL16, HFH11, MPP2, WIN; Forkhead box protein M1; Forkhead-related protein FKHL16; Hepatocyte nuclear factor 3 forkhead homolog 11 (HFH-11; HNF-3/fork-head homolog 11); M-phase phosphoprotein 2; MPM-2 reactive phosphoprotein 2; Transcription factor Trident; Winged-helix factor from INS-1 cells
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
FOXM1 Antibody (YA1112)
目录号:
HY-P81367
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