2. 抗体
  3. 一抗
  4. 多克隆抗体
  5. GPR43 抗体

GPR43 Antibody 是一个兔来源、无偶联标记、抗 GPR43 的 IgG 多克隆抗体。

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规格 价格 是否有货 数量
20 μL ￥846 In-stock
50 μL ￥1250 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

GPR43 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GPR43.
宿主

Rabbit
克隆性

Polyclonal
分子量
Predicted band size: 37 kDa;
Observed band size: 37 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse 预测反应种属: Rat
请注意：预测反应种属仅供参考，不作为质保凭证。
蛋白数据库
基因 ID
免疫原

KLH conjugated synthetic peptide derived from human GPR43: 41-140/330
应用 & 推荐
稀释比例
应用 稀释比
ELISA
ELISA: 酶联免疫吸附试验
1:5000-10000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-500
IHC-F
IHC-F: 冰冻切片样本的免疫组织化学
1:100-500
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-500
IF-Tissue
IF-Tissue: 组织免疫荧光
1:100-500
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体
组分

1.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
2.Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Please refer to the lot-specific COA for specific buffer information.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL IHC mIHC

  • Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using GPR43 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81225, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using GPR43 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81225, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using GPR43 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81225, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil tissue using GPR43 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81225, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Tonsil‌ tissue using GPR43 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81225, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using GPR43 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81225, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using GPR43 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81225, 1：200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using GPR43 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81225, 1：200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using GPR43 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81225, 1：200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using GPR43 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81225, 1：200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using GPR43 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81225, 1：200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Tonsil tissue using GPR43 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81225, 1：200 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景
功能：G 蛋白偶联受体可被膳食纤维消化的主要产物——短链脂肪酸 (SCFAs) 激活，并在全身能量稳态调节和肠道免疫中发挥作用。在杂食性哺乳动物中，短链脂肪酸乙酸、丙酸和丁酸主要由代谢膳食纤维的肠道微生物群产生。SCFAs 既是能量来源，也作为信号分子发挥作用。该 G 蛋白偶联受体可能与百日咳毒素敏感的 G (i/o)-α家族 G 蛋白偶联，也可能与 Gq 家族 G 蛋白偶联 (PubMed:12496283, PubMed:12711604, PubMed:23589301)。其激活可导致肌醇 1,4,5-三磷酸的生成、细胞内钙的动员、MAPK3/ERK1 和 MAPK1/ERK2 激酶的磷酸化以及细胞内 cAMP 积累的抑制。它可能通过调节 GLP-1 的分泌来维持葡萄糖稳态，以响应肠道中短链脂肪酸的积累。它还可能调节瘦素 (LEP/Leptin) 的产生，瘦素是一种作用于中枢神经系统以抑制食物摄入的激素。最后，它还可能通过调节脂肪细胞的分化和脂质储存，进而调节全身能量稳态。除了在能量稳态中的作用外，它还可能介导肠道中短链脂肪酸激活炎症和免疫反应，调节趋化因子和细胞因子的快速产生。它还可能在炎症反应的消退和中性粒细胞趋化性的调控中发挥作用。除了短链脂肪酸 (SCFAs) 外，该受体还可能被细胞外凝集素 FCN1 激活，从而激活单核细胞并诱导其在微生物存在下分泌白细胞介素-8/IL-8 (PubMed:21037097)。在 SCFAs (碳原子数少于 6 个的脂肪酸) 中，乙酸、丙酸和丁酸可能是最有效的激活剂 (PubMed:12496283, PubMed:12711604)。该受体表现出不依赖于 SCFAs 的组成型 G 蛋白偶联受体活性 (PubMed:23066016)。
亚细胞定位：细胞膜；多通道膜蛋白
表达水平：
组织特异性：在外周血白细胞中表达水平相对较高，在脾脏中表达水平较低。
亚基：与 FCN1 相互作用 (通过纤维蛋白原 C 端结构域)
RRID
反应种属数据库

Entrez Gene: 2867 Human ; 233079 Mouse ;

SwissProt: O15552 Human ; Q8VCK6 Mouse ;

OMIM: 603823 Human

中文名
G蛋白偶联受体43抗体
同用名
GPCR GPR43; FFA2R; Ffar2; FFAR2_HUMAN; free fatty acid activated receptor 2; Free fatty acid receptor 2; G protein coupled receptor 43; G protein-coupled receptor 43; G-protein coupled receptor 43; GPR 43; GPCR 43.
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

GPR43 Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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