1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. Integrin beta 4 抗体 (YA3280)

Integrin beta 4 Antibody (YA3280) 是一个兔来源、无偶联标记、抗 Integrin beta 4 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥420 In-stock
50 μL ¥1102 In-stock
100 μL ¥1732 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Integrin beta 4 Antibody (YA3280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Integrin beta 4.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 202 kDa;
Observed band size: 210 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human Integrin beta 4 aa1600-1822.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
敏感性 Endogenous 纯度 Affinity Chromatography
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
IHC-P
  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Integrin beta 4 Antibody (YA3280). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Integrin beta 4 Antibody (YA3280). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83535, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:整合素α-6/β-4 是层粘连蛋白的受体,在上皮细胞的半桥粒中发挥着关键的结构作用,并且是调节角质形成细胞极性和运动所必需的。ITGA6:ITGB4 通过其 EGF 结构域与 NRG1 结合,这种结合对于 NRG1-ERBB 信号通路至关重要 (PubMed:20682778)。ITGA6:ITGB4 与 IGF1 结合,这种结合对于 IGF1 信号通路至关重要 (PubMed:22351760)。ITGA6:ITGB4 与 IGF2 结合,这种结合对于 IGF2 信号通路至关重要 (PubMed:28873464)。
亚细胞定位:细胞膜;单次跨膜 I 型膜蛋白;细胞膜;脂锚;细胞连接,半桥粒
表达水平:
组织特异性:整合素α6/β4 主要由上皮细胞表达。β4D 亚型也在结肠和胎盘中表达。β4E 亚型也在表皮、肺、十二指肠、心脏、脾脏和胃中表达。
异构体 & 翻译后修饰:P16144 有 5 种异构体:P16144-1:202167 Da (预测值);P16144-2:195013 Da (预测值);P16144-3:200725 Da (预测值);P16144-4:194466 Da (预测值);P16144-5:106779 Da (预测值)。
P16144 在膜近端区域的多个半胱氨酸残基上被 DHHC3 棕榈酰化,从而增强其稳定性和细胞表面表达。棕榈酰化还促进其与四跨膜蛋白的二级结合。
亚基:由α亚基和β亚基组成的异二聚体。β-4 与α-6 结合。通过胞质区与 COL17A1 相互作用。通过胞质区与 DST 亚型 3 相互作用 (通过 N 端)。亚型β-4a 通过胞质结构域与 DST 相互作用 (通过 N 端)。与 RAC1 相互作用。ITGA6:ITGB4 与 NRG1 和 ERBB3 形成三元复合物 (PubMed:20682778)。ITGA6:ITGB4 与 IGF1 和 IGF1R 形成三元复合物 (PubMed:22351760)。ITGA6:ITGB4 与 IGF2 相互作用 (PubMed:28873464)。与 TMEM268 相互作用。这种相互作用可防止 ITGB4 降解 (PubMed:30361615)
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
Integrin beta 4 抗体 (YA3280)
同用名
CD104; gp150; Integrin beta 4 subunit; ITG B4; ITGB 4; Itgb4
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Integrin beta 4 Antibody (YA3280)
目录号:
HY-P83535
需求量: