2. 抗体
  3. 一抗
  4. 单克隆抗体 流式抗体
  5. MUC2 抗体 (YA4171)

MUC2 Antibody (YA4171) 是一个小鼠来源、无偶联标记、抗 MUC2 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥609 In-stock
50 μL ￥1575 In-stock
100 μL ￥2520 In-stock
250 μL   询价  

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

MUC2 Antibody (YA4171) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MUC2.
宿主

Mouse
克隆性

Monoclonal
分子量
Predicted band size: 540 kDa;
Observed band size: 110 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse, Rat, Rabbit
蛋白数据库
基因 ID
免疫原

Purified recombinant fragment of human MUC2 (AA: 4373-4557) expressed in E. Coli.
应用 & 推荐
稀释比例
应用 稀释比
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:200-1:1000
FC
FC: 流式细胞术
1:200-1:400
ELISA
ELISA: 酶联免疫吸附试验
1:10000
纯度 affinity purified. 偶联 Non-conjugated
修饰 Unmodified 同型 IgG1
性状

液体
组分

Supplied in PBS with 0.05% sodium azide
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
IHC-P

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using MUC2 Antibody antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84474, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue using MUC2 Antibody antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84474, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using MUC2 Antibody antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84474, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Colorectal Cancer 1 tissue using MUC2 Antibody antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84474, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Colorectal Cancer 2 tissue using MUC2 Antibody antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84474, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human Colorectal Cancer 3 tissue using MUC2 Antibody antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked with QuickBlock at room temperature for 30 minutes, washed with PBSand PBST, and then incubated with the primary antibody (HY-P84474, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能：覆盖于肠道和其他含黏膜器官的上皮细胞表面，形成一道保护性润滑屏障，抵御黏膜表面的颗粒物和病原体 (PubMed:17058067, PubMed:19432394, PubMed:33031746)。它是结肠黏液的主要成分，结肠黏液主要由覆盖肠道暴露表面的杯状细胞分泌的大型 MUC2 聚合物网络构成 (PubMed:19432394, PubMed:33031746)。MUC2 网络形成水凝胶，保护下方的上皮细胞免受病原体和其他有害物质的侵害，同时允许营养物质的吸收和气体交换 (PubMed:33031746, PubMed:36206754)。 MUC2 作为一种二价铜分子伴侣，能够保护肠道细胞免受铜毒性侵害，并促进细胞对营养性铜的吸收 (PubMed:36206754)。它能结合 Cu (2+) 及其还原态 Cu (1+)，结合位点有两个相邻的位点：Cu (2+) 一旦被维生素 C (抗坏血酸) 或其他膳食抗氧化剂还原为 Cu (1+)，便会转移到另一个结合位点 (PubMed:36206754)。在有氧环境下，MUC2 结合的 Cu (1+) 可免受氧化，并可释放出来为细胞提供营养 (PubMed:36206754)。黏蛋白凝胶储存着参与先天免疫的抗菌分子 (PubMed:33031746)。黏蛋白糖蛋白不仅为肠道微生物群提供栖息和营养，还能润滑组织表面，并可能促进体内污染物和代谢废物的清除 (PubMed:33031746)。杯状细胞合成两种形式的 MUC2 黏蛋白，它们的区别在于支链 O-糖基化和在结肠中的产生部位：一种是 (1)“浓稠”的黏液，包裹肠道微生物群形成粪便颗粒，产生于近端升结肠 (基于相似性)。这种“浓稠”的黏液沿降结肠流动，并被一种 (2)“稀薄”的 MUC2 黏液润滑，后者产生于远端结肠，并粘附于“浓稠”的黏液上 (基于相似性)。
亚细胞定位：分泌物
表达水平：
组织特异性：结肠、小肠、结肠肿瘤、支气管、宫颈和胆囊
亚基：同源多聚体；二硫键连接 (PubMed:12374796, PubMed:31310764, PubMed:33031746, PubMed:35377815)。N 端和 C 端介导其组装成更高级的结构，形成丝状体 (PubMed:33031746, PubMed:35377815)。两个多肽的 CTCK 结构域在内质网中结合，形成分子间二硫键连接的二聚体 (基于相似性)。这些二聚体进入高尔基体，高尔基体的环境比内质网更酸性 (PubMed:33031746)。在酸性条件下，N 端形成非共价分子间相互作用，使来自不同 CTCK 连接的二聚体的第三 VWD 结构域 (VWD3) 组装体并置 (PubMed:33031746)。VWD3 组装体随后通过二硫键相互连接，形成长链的二硫键连接的聚合物，这些聚合物在分泌前保持高度紧密的结构 (PubMed:33031746)。与 FCGBP 相互作用 (PubMed:19432394)。与 AGR2 相互作用；形成二硫键连接 (PubMed:19359471)。
RRID
中文名
MUC2 抗体
同用名
MLP; SMUC; MUC-2
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

MUC2 Antibody (YA4171) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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