PAK2 抗体 (YA695)

目录号: HY-P80778 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
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PAK2 Antibody (YA695) 是一个小鼠来源、无偶联标记、抗 PAK2 的 IgG1 单克隆抗体。

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规格	价格	是否有货
10 μL	￥ 320.45 ~~￥493.00~~	In-stock
50 μL	￥ 832.65 ~~￥1281.00~~	In-stock
100 μL	￥ 1365.00 ~~￥2100.00~~	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

产品详情
验证图片
背景信息
产品资料

描述

PAK2 Antibody (YA695) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PAK2.

宿主

Mouse

克隆性

Monoclonal

分子量

Predicted band size: 58 kDa;

Observed band size: 61 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Monkey

蛋白数据库

Q13177

基因 ID

5062 [NCBI]

免疫原

Synthetic peptide corresponding tofragment of PAK2.The exact sequence is proprietary to MCE.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:100
IHC-F IHC-F: 冰冻切片样本的免疫组织化学	1:50-1:100
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200

敏感性	Endogenous	纯度	affinity purified
偶联	Non-conjugated	修饰	Unmodified
同型	IgG1

性状

液体

组分

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

IHC

Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using PAK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80778, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using PAK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80778, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using PAK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80778, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using PAK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80778, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using PAK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80778, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using PAK2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80778, 1:50 dilution) at room temperature for Leave overnight at 4°C. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

背景

功能：丝氨酸/苏氨酸蛋白激酶，参与多种不同的信号通路，包括细胞骨架调控、细胞运动、细胞周期进程、细胞凋亡或细胞增殖 (PubMed:12853446, PubMed:16617111, PubMed:19273597, PubMed:19923322, PubMed:33693784, PubMed:7744004, PubMed:9171063)。它是小 GTP 酶 CDC42 和 RAC1 的下游效应分子 (PubMed:7744004)。活性 CDC42 和 RAC1 的结合激活该激酶，导致其构象变化，并随后在多个丝氨酸和/或苏氨酸残基上发生自身磷酸化 (PubMed:7744004)。全长 PAK2 可促进细胞存活和生长 (PubMed:7744004)。它能磷酸化 MAPK4 和 MAPK6，并激活下游靶标 MAPKAPK5，后者是 F-肌动蛋白聚合和细胞迁移的调节因子 (PubMed:21317288)。它还能磷酸化 JUN，并在 EGF 诱导的细胞增殖中发挥重要作用 (PubMed:21177766)。此外，它还能磷酸化多种其他底物，包括组蛋白 H4，以促进 H3.3 和 H4 组装成核小体，以及 BAD、核糖体蛋白 S6 或 MBP (PubMed:21724829)。它还能磷酸化 CASP7，从而抑制其活性 (PubMed:21555521, PubMed:27889207)。此外，PAK2 与 ARHGEF7 和 GIT1 结合，执行一些不依赖于激酶活性的功能，例如在有丝分裂过程中控制纺锤体方向 (PubMed:19273597, PubMed:19923322)。另一方面，凋亡刺激，例如 DNA 损伤，会导致 caspase 介导的 PAK2 裂解，生成活性 p34 片段 PAK-2p34，该片段易位至细胞核，并通过 JNK 信号通路促进细胞凋亡 (PubMed:12853446, PubMed:16617111, PubMed:9171063)。Caspase 激活的 PAK2 可磷酸化 MKNK1 并降低细胞翻译 (PubMed:15234964)。

亚细胞定位：细胞质；细胞核；细胞核；细胞质，核周区域；膜；脂锚

表达水平：
组织特异性：广泛表达。在骨骼肌、卵巢、胸腺和脾脏中表达水平较高。

亚基：PAK-2p34 与 GTP 结合的 CDC42/p21 和 RAC1 紧密相互作用，但不与 GDP 结合的 CDC42/p21 和 RAC1 相互作用 (PubMed:20696164)。PAK-2p34 与 SH3MD4 相互作用 (PubMed:16374509)。PAK-2p34 与 SCRIB 相互作用 (PubMed:18716323)。PAK-2p34 与 ARHGEF7 和 GIT1 相互作用 (PubMed:19273597)。PAK-2p34 与 ARHGAP10 相互作用 (PubMed:15471851)。

RRID

AB_3102189

反应种属数据库

Entrez Gene: 5062 Human ;

SwissProt: Q13177 Human ;

OMIM: 618458 Human

研究领域

Cell Biology

中文名

PAK2 抗体 (YA695)

同用名

PAK2; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

PAK2 Antibody (YA695) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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