2. 抗体
  3. 一抗
  4. 单克隆抗体 流式抗体
  5. PD-1 抗体(YA3401)

PD-1 Antibody(YA3401) 是一个小鼠来源、无偶联标记、抗 PD-1 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥525 In-stock
50 μL ￥1365 In-stock
100 μL ￥2205 In-stock
250 μL   询价  

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

PD-1 Antibody(YA3401) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PD-1.
宿主

Mouse
克隆性

Monoclonal
分子量
Predicted band size: 32 kDa;
Observed band size: 32 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human
蛋白数据库
基因 ID
免疫原

Recombinant protein within Human PD1 aa 1-200 / 288.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:2000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:100-1:500
FC
FC: 流式细胞术
1:50-1:100
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG1  
性状

液体
组分

Supplied in 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
IHC

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue(negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (negative) using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using PD-1 Antibody(YA3401). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81169A,1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

背景
功能：抗原激活的 T 细胞上的抑制性受体在诱导和维持自身免疫耐受中起着关键作用 (PubMed:21276005, PubMed:37208329)。PDCD1 与配体 CD274/PDCD1L1 和 CD273/PDCD1LG2 结合后，传递抑制信号 (PubMed:21276005)。T 细胞受体 (TCR) 激活后，PDCD1 与免疫突触中的 CD3-TCR 结合，并直接抑制 T 细胞活化 (基于相似性)。 PDCD1 通过募集 PTPN11/SHP-2 抑制 T 细胞活化：配体结合后，PDCD1 的 ITSM 基序发生磷酸化，进而募集蛋白酪氨酸磷酸酶 PTPN11/SHP-2，后者介导关键 TCR 近端信号分子 (如 ZAP70、PRKCQ/PKCθ和 CD247/CD3ζ) 的去磷酸化 (基于相似性)。肿瘤利用 PDCD1 介导的抑制通路来减弱抗肿瘤免疫反应并逃避免疫系统的清除，从而促进肿瘤存活 (PubMed:28951311)。PDCD1 与 CD274/PDCD1L1 的相互作用抑制细胞毒性 T 淋巴细胞 (CTL) 的效应功能 (PubMed:28951311)。阻断 PDCD1 介导的通路可逆转耗竭 T 细胞表型并使抗肿瘤反应正常化，为癌症免疫疗法提供了理论依据 (PubMed:22658127, PubMed:25034862, PubMed:25399552)。
亚细胞定位：细胞膜；单次跨膜 I 型膜蛋白
亚基：单体 (PubMed：26602187)。与 CD274/PDCD1L1 相互作用 (PubMed：26602187)。与 CD273/PDCD1LG2 相互作用 (基于相似性)。与 FBXO38 相互作用；导致 PDCD1 泛素化并被蛋白酶体降解 (PubMed：30487606)。
RRID
反应种属数据库

Entrez Gene: 5133 Human

SwissProt: Q15116 Human

OMIM: 605218 Human

中文名
程序性死亡1（CD279）抗体
同用名
Programmed cell death protein 1; CD279; CD279 antigen; hPD 1; hPD-1; hSLE1; PD 1; PD1; PDCD 1; PDCD1; PDCD1_HUMAN; Programmed cell death 1; Protein PD 1; Protein PD-1; SLEB2; Systemic lupus erythematosus susceptibility 2.
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

PD-1 Antibody(YA3401) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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