VGLUT2 抗体 (YA6832)

目录号: HY-P87139 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
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VGLUT2 Antibody (YA6832) 是一个兔来源、无偶联标记、抗 VGLUT2 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥525	In-stock
50 μL	￥1365	In-stock
100 μL	￥2205	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

产品详情
验证图片
背景信息
产品资料

描述

VGLUT2 Antibody (YA6832) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to VGLUT2.

宿主

Rabbit

克隆性

Recombinant,Monoclonal

分子量

Predicted band size: 65 kDa;

Observed band size: 65 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Mouse, Rat 预测反应种属: Cynomolgus monkey,Pig

请注意：预测反应种属仅供参考，不作为质保凭证。

蛋白数据库

Q8BLE7

基因 ID

140919 [NCBI]

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:2000
IHC-F IHC-F: 冰冻切片样本的免疫组织化学	1:500
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:2000

纯度	affinity purified.	偶联	Non-conjugated
修饰	Unmodified	同型	IgG

性状

液体

组分

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL IHC mIHC

Immunohistochemical analysis of paraffin-embedded Rat Brain tissue using VGLUT2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87139, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded Rat Brain tissue using VGLUT2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87139, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded Rat Brain tissue using VGLUT2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87139, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded Rat Brain tissue using VGLUT2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87139, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded Rat Brain tissue using VGLUT2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87139, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Immunohistochemical analysis of paraffin-embedded Rat Brain tissue using VGLUT2 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87139, 1:300 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Rat Brain tissue using VGLUT2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87139, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Rat Brain tissue using VGLUT2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87139, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Rat Brain tissue using VGLUT2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87139, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Rat Brain tissue using VGLUT2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87139, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Rat Brain tissue using VGLUT2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87139, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded Rat Brain tissue using VGLUT2 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87139, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景

功能：多功能转运蛋白，可转运 L-谷氨酸以及多种离子，例如氯离子、质子、钾离子、钠离子和磷酸根离子 (PubMed:11432869, PubMed:17108179, PubMed:25433636, PubMed:33440152)。在突触小泡膜上，它主要作为单向转运蛋白发挥作用，优先将 L-谷氨酸以及磷酸根离子从细胞质转运到兴奋性神经细胞突触前神经末梢的突触小泡中 (PubMed:11432869, PubMed:17108179)。 L-谷氨酸或磷酸盐单向转运蛋白的活性是电致性的，由质子电化学梯度驱动，主要由突触小泡膜上液泡 H⁺-ATPase 建立的电位梯度驱动 (PubMed:11432869)。此外，它还作为氯离子通道，允许氯离子穿过突触小泡膜，从而影响质子电化学梯度并促进突触小泡酸化 (基于相似性)。此外，它还作为囊泡 K⁺/H⁺反向转运蛋白，维持电位梯度并降低化学梯度，从而维持囊泡对谷氨酸的摄取 (PubMed:25433636)。囊泡 H⁺/H⁺反向转运蛋白的活性是电中性的 (PubMed:25433636)。在胞吐作用后，该蛋白在质膜上作为钠离子 (Na⁺) 和磷酸盐的同向转运蛋白，将细胞外空间的钠离子转运至细胞质，从而调节突触磷酸盐稳态 (PubMed:33440152)。该同向转运蛋白的活性由膜内负电位驱动，且具有电生成特性 (PubMed:33440152)。此外，该蛋白还参与出生后发育过程中视网膜玻璃体血管的退化调节 (PubMed:30936473)。基于相似性推测，该蛋白可能在其他组织 (如松果体和胰腺) 的内分泌谷氨酸能系统中发挥作用。

亚细胞定位：细胞质囊泡、分泌囊泡、突触囊泡膜、突触、突触体、细胞膜

表达水平：
组织特异性：在大脑中表达。在海马神经元中表达 (蛋白质水平)。

异构体 & 翻译后修饰：Q8BLE7：氨基酸个数 582 个，分子量为 64561 Da。

RRID

AB_3719270

中文名

VGLUT2 抗体 (YA6832)

同用名

Differentiation associated BNPI antibody; Differentiation associated Na dependent inorganic phosphate cotransporter antibody; Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody; Differentiation-associated BNPI antibody; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody; DNPI antibody; SLC17A6 antibody; Sodium dependent inorganic phosphate cotransporter antibody; Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody; Solute carrier family 17 member 6 antibody; Differentiation associated BNPI antibody; Differentiation associated Na dependent inorganic phosphate cotransporter antibody; Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody; Differentiation-associated BNPI antibody; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody; DNPI antibody; SLC17A6 antibody; Sodium dependent inorganic phosphate cotransporter antibody; Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody; Solute carrier family 17 member 6 antibody; Vesicular glutamate transporter 2 antibody; Vesicular glutamate transporter type 2 antibody; VGLU2_HUMAN antibody; VGLUT 2 antibody; VGluT2 antibody;

文件资料

Select Batch:

Data Sheet (440 KB) SDS (254 KB)

COA (194 KB)

抗体使用指南 (1083 KB)

VGLUT2 Antibody (YA6832) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

粤ICP备2021105330号-3

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

VGLUT2 抗体 (YA6832)

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VGLUT2 抗体 (YA6832)

实现从蛋白分离到检测的高效衔接！

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GelHP 蛋白预制胶 (Bis-Tris, 10%, 10 孔)

GelHP 蛋白预制胶 (Bis-Tris, 10%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 12%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 15%, 10 孔)

GelHP 蛋白预制胶 (Bis-Tris, 15%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 4-15%, 10 孔)

GelHP 蛋白预制胶 (Bis-Tris, 4-15%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 4-20%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 8-16%, 10 孔)

GelHP 蛋白预制胶 (Bis-Tris, 8-16%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 8-20%, 15 孔)

GelHP 蛋白预制胶 (Tris-Gly, 8%, 10 孔)

GelHP 蛋白预制胶 (Tris-Gly, 8%, 15 孔)

GelHP 蛋白预制胶 (Bis-Tris, 8%, 10 孔)

GelHP 蛋白预制胶 (Bis-Tris, 8%, 15 孔)

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