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  3. Fluorescent Dye
  4. CTAP-1

CTAP-1 是一种一价铜离子探针。CTAP-1 可与一价铜 Cu(I) 结合以抑制光诱导电子转移，从而增强荧光发射。CTAP-1 可产生铜依赖性核周染色，该染色与线粒体及高尔基体共定位。CTAP-1 可用于对 Cu(I) 库进行成像。

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CTAP-1

CTAP-1 Chemical Structure

CAS No. : 866534-64-7

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CTAP-1 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

CTAP-1 is a monovalent copper ion probe. CTAP-1 binds to monovalent copper Cu (I) to inhibit photoinduced electron transfer, thereby enhancing fluorescence emission. CTAP-1 produces copper-dependent perinuclear staining that colocalizes with mitochondria and the Golgi apparatus. CTAP-1 can be used for imaging Cu (I) pools[1].

体外研究
(In Vitro)

CTAP-1 (10 μM; 50 min) 可标记 NIH 3T3 成纤维细胞中核周的铜依赖性不稳定铜池；在铜补充培养基中其荧光强度升高约 2 至 3 倍，且染色结果与线粒体和高尔基体共定位[1]。
CTAP-1 (2 μM) 在铜负载的 NIH 3T3 成纤维细胞中的荧光经外源性 Cu+螯合剂 DTO 处理后降低约 50%，证实该信号具有铜依赖性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CTAP-1 相关抗体:

Immunofluorescence[1]

Cell Line: mouse NIH 3T3 fibroblast cells
Concentration: 10 μM (standard staining; immunofluorescence colocalization); 150 μM CuCl2 (cell pre-culture)
Incubation Time: 50 min (37 °C, DMEM for standard staining; room temperature, PBS for immunofluorescence colocalization); 12 h (cell pre-culture with CuCl2)
Result: Produced a weak perinuclear staining pattern in basal medium.
Showed an ~2- to 3-fold stronger emission intensity in cells pre-cultured with 150 μM CuCl2 for 12 h.
Colocalized staining pattern with subcellular locations of the Golgi apparatus and mitochondria.
分子量

623.92

Formula

C32H37N3O2S4
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

CTAP-1 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CTAP-1866534-64-7CTAP1CTAP 1Fluorescent Dyemonovalent copper ion probeInhibitorinhibitorinhibit

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