2. Others Apoptosis
  3. Fluorescent Dye Caspase
  4. CyGbPF

CyGbPF 是一种颗粒酶 B (granzyme B) 特异性近红外荧光探针。CyGbPF 可被颗粒酶 B 切割以去除肽笼状基团，恢复近红外荧光。CyGbPF 可被动蓄积于小鼠肿瘤中，其激活荧光与肿瘤组织中颗粒酶 B 的表达、CD8+ 细胞毒性 T 淋巴细胞群及 CD4+ 辅助性 T 淋巴细胞群相关。CyGbPF 可被肾脏高效清除，从而能够通过光学尿液分析评估免疫激活情况。CyGbPF 可对活体动物的癌症免疫效果进行实时无创评估。CyGbPF 可用于乳腺癌等癌症的研究。

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CyGbPF

CyGbPF Chemical Structure

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CyGbPF 相关抗体

Top Publications Citing Use of Products

查看 Caspase 亚型特异性产品:

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

CyGbPF is a granzyme B-specific near-infrared fluorescent probe. CyGbPF can be cleaved by granzyme B to remove the peptide caging group, restoring near-infrared fluorescence. CyGbPF passively accumulates in mouse tumors, and its activated fluorescence correlates with granzyme B expression, CD8+ cytotoxic T lymphocyte populations, and CD4+ helper T lymphocyte populations in tumor tissues. CyGbPF is efficiently cleared by the kidneys, enabling the assessment of immune activation via optical urine analysis. CyGbPF allows real-time non-invasive evaluation of cancer immunotherapeutic efficacy in living animals. CyGbPF can be used in the research of cancers such as breast cancer[1].

体外研究
(In Vitro)

CyGbPF (2-250 μM; 5-90 min) 可在无细胞实验中被小鼠颗粒酶 B 特异性切割，表现出 24 倍的近红外荧光增强[1]。
CyGbPF (5 μM; 0.5-2.0 h; 1.60-200 μg/mL) 可被 CD8+ T 细胞中的内源性颗粒酶 B 快速激活，其时间依赖性荧光增强幅度较 4T1 细胞高约 35 倍，较 RAW264.7 细胞高约 15 倍；且在这三种细胞系中，浓度高达 200 μg/mL 时仍仅表现出极低的细胞毒性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CyGbPF 相关抗体:
体内研究
(In Vivo)

CyGbPF (10 μM/kg; 静脉注射; 单次给药) 在乳腺癌小鼠中可产生肿瘤局部的、依赖激活的 NIRF 信号，该信号与免疫激活水平相关；可通过肿瘤 NIRF 成像和光学尿液分析实现对分级免疫激活的无创追踪[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c (tumor-bearing, implanted with 4T1 breast cancer cells, pretreated with immunotherapeutic agents for immunoactivation)[1]
Dosage: 10 μM/kg
Administration: i.v.; single dose
Result: Reached maximum tumor NIRF signal intensity at 6 hours post-injection.
Showed tumor NIRF signals 1.47-fold (NLG919-pretreated), 1.54-fold (R848-pretreated), 2.23-fold (BMS-1-pretreated), 2.79-fold (pexidartinib-pretreated), and 2.85-fold (BEC-pretreated) higher than control mice at 6 hours post-injection.
Accumulated 14% of injected dose in tumors with negligible accumulation in other organs.
Correlated strongly with tumor tissue levels of granzyme B, CD8+ T cells, and CD4+ T cells, which were elevated in immunotherapy-treated mice relative to controls.
分子量

3367.84

Formula

C163H273IN8O56
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

CyGbPF 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CyGbPFFluorescent DyeCaspaseCD8+ T cellsbreast cancerCD4+ T helper cell populations4T1 cellsmouse granzyme BRAW264.7 cellsmouse tumorsgranzyme B (mouse)CD8+ cytotoxic T lymphocyte populationsInhibitorinhibitorinhibit

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