2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. LR004

LR004 是一种 EGFR 单克隆抗体，与人 EGFR 结合的 Kd 值为 2.80 × 10-9 M。LR004 在体外对 EGFR 阳性肿瘤细胞的活力抑制作用极弱，但单药使用时可抑制 EGFR 阳性肿瘤异种移植物的生长。LR004 适用于晚期结直肠癌、实体瘤、食管鳞状细胞癌、表皮样癌及乳腺癌相关研究。

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LR004

LR004 Chemical Structure

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LR004 相关抗体

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

LR004 is an EGFR monoclonal antibody, with a Kd of 2.80 × 10-9 M against human EGFR. LR004 shows extremely weak inhibitory effect on the viability of EGFR-positive tumor cells in vitro, but inhibits the growth of EGFR-positive tumor xenografts as a single agent. LR004 is applicable to research related to advanced colorectal cancer, solid tumors, esophageal squamous cell carcinoma, epidermoid carcinoma, colon cancer and breast cancer[1][2].

反应种属

Human
体外研究
(In Vitro)

LR004 (5.0 μg/mL) 以中等亲和力结合重组人 EGFR 抗原，其 Kd 为 2.80 × 10-9 M[1]。
LR004 (0.023-50 μg/mL; 24 h initial incubation, followed by 96 h incubation with serum-containing medium) 可抑制人乳腺癌 MDA-MB-468 细胞的增殖，其 EC50 为 2.278 μg/mL[1]。
LR004 (0.001-1 μg·mL-1; 48 h) 对人源 EGFR 阳性肿瘤细胞 (KYSE520、KYSE150、A431、HCC827、NCI-H1975、A549、MDA-MB-468、MCF-7、AsPC-1) 和 EGFR 阴性 Karpas 299 细胞具有微弱的 in vitro 细胞毒性，对应的 IC50 值分别>6 nM 或>60 nM[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

LR004 相关抗体:

Cell Proliferation Assay[1]

Cell Line: MDA-MB-468 human breast cancer cells
Concentration: 0.023-50 μg/mL
Incubation Time: 24 h (initial incubation); 96 h (incubation with serum-containing medium)
Result: Inhibited MDA-MB-468 cell proliferation with an EC50 of 2.278 μg/mL.

Cell Cytotoxicity Assay[2]

Cell Line: KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, AsPC-1, Karpas 299
Concentration: 0.001-1 μg·mL-1
Incubation Time: 48 h
Result: Had a weak inhibitory effect on cell viability, with IC50 values >6 nM for KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, and AsPC-1 cells, and IC50 >60 nM for Karpas 299 cells.
体内研究
(In Vivo)

LR004 (15 mg/kg; i.v.; every 4 days; 4 total doses) 在 KYSE520 食管鳞状细胞癌异种移植物中仅诱导轻微的肿瘤生长抑制，肿瘤抑制率为 13.8%[2]。
LR004 (15 mg/kg; i.v.; every 4 days; 6 total doses) 在 A431 表皮样癌异种移植物中仅能发挥有限的肿瘤生长抑制作用[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude (female, 6-8 weeks old, 18-20 g, KYSE520 tumor cell xenograft)[2]
Dosage: 15 mg/kg
Administration: i.v.; every 4 days; 4 total doses
Result: Achieved an average tumor volume of 1791 mm3 on day 44.
Induced a 13.8% tumor inhibition rate.
Showed no tumor regression.
Caused no body weight loss or mortality.
Detected no histopathological abnormalities in major organs.
Animal Model: BALB/c nude (female, 6-8 weeks old, 18-20 g, A431 tumor cell xenograft)[2]
Dosage: 15 mg/kg
Administration: i.v.; every 4 days; 6 total doses
Result: Achieved an average tumor volume of 399 mm3 on day 60.
Exhibited limited therapeutic activity compared to LR004-VC-MMAE groups.
Detected no histopathological abnormalities in major organs.
基因 ID

1956  [NCBI]

Accession

P00533-1
靶点

EGFR
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

LR004 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

LR004LR 004LR-004EGFREpidermal growth factor receptorErbB-1HER1rhesus monkeysMDA-MB-468 human breast cancer cellsA431 xenograft tumorsnude miceKYSE520 cellscolorectal carcinomalysosomesxenograft micehuman EGFRInhibitorinhibitorinhibit

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目录号：
HY-P991969
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