2. Academic Validation
  3. Photoactivation of silicon rhodamines via a light-induced protonation

Photoactivation of silicon rhodamines via a light-induced protonation

  • Nat Commun. 2019 Oct 8;10(1):4580. doi: 10.1038/s41467-019-12480-3.
Michelle S Frei 1 2,  Philipp Hoess 3 4,  Marko Lampe 5,  Bianca Nijmeijer 3,  Moritz Kueblbeck 3,  Jan Ellenberg 3,  Hubert Wadepohl 6,  Jonas Ries 3,  Stefan Pitsch 7,  Luc Reymond 8 9,  Kai Johnsson 10 11 12
Affiliations

Affiliations

  • 1 Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.
  • 2 Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
  • 3 Cell Biology and Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
  • 4 Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, Germany.
  • 5 Advanced Light Microscopy Facility (ALMF), European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117, Heidelberg, Germany.
  • 6 Anorganisch-Chemisches Institut, University of Heidelberg, Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.
  • 7 Spirochrome AG, Chalberweidstrasse 4, CH-8260, Stein am Rhein, Switzerland.
  • 8 Biomolecular Screening Facility, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland. luc.reymond@epfl.ch.
  • 9 National Centre of Competence in Research (NCCR) in Chemical Biology, 1015, Lausanne, Switzerland. luc.reymond@epfl.ch.
  • 10 Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany. johnsson@mr.mpg.de.
  • 11 Institute of Chemical Sciences and Engineering (ISIC), École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland. johnsson@mr.mpg.de.
  • 12 National Centre of Competence in Research (NCCR) in Chemical Biology, 1015, Lausanne, Switzerland. johnsson@mr.mpg.de.
PMID: 31594948 DOI: 10.1038/s41467-019-12480-3
Abstract

Photoactivatable fluorophores are important for single-particle tracking and super-resolution microscopy. Here we present a photoactivatable fluorophore that forms a bright silicon rhodamine derivative through a light-dependent protonation. In contrast to Other photoactivatable fluorophores, no caging groups are required, nor are there any undesired side-products released. Using this photoactivatable fluorophore, we create probes for HaloTag and actin for live-cell single-molecule localization microscopy and single-particle tracking experiments. The unusual mechanism of photoactivation and the fluorophore's outstanding spectroscopic properties make it a powerful tool for live-cell super-resolution microscopy.

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