2. Academic Validation
  3. A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals

A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals

  • Bioconjug Chem. 2019 Nov 20;30(11):2958-2966. doi: 10.1021/acs.bioconjchem.9b00643.
Hong-Shan Liu 1,  Takumi Ishizuka 1,  Makiko Kawaguchi 2,  Ryuichi Nishii 3,  Hiroaki Kataoka 2,  Yan Xu 1
Affiliations

Affiliations

  • 1 Division of Chemistry, Department of Medical Sciences, Faculty of Medicine , University of Miyazaki , 5200 Kihara , Kiyotake , Miyazaki 889-1692 , Japan.
  • 2 Section of Oncopathology and Regenerative Biology, Department of Pathology, Faculty of Medicine , University of Miyazaki , Miyazaki 889-1692 , Japan.
  • 3 Department of Molecular Imaging and Theranostics, National Institute of Radiological Sciences (NIRS) , National Institutes for Quantum and Radiological Science and Technology (QST) , Chiba 263-8555 , Japan.
PMID: 31638370 DOI: 10.1021/acs.bioconjchem.9b00643
Abstract

In the present study, we used a nucleoside derivative 5-vinyluridine (VrU) for labeling during cell division and for tumor imaging in living mice. We demonstrated that the functional nucleoside bearing a 5-vinyl group is metabolically incorporated into cellular RNA and can be used to image RNA using a Diels-Alder reaction. The reagent allows for simultaneous and clear imaging of DNA and RNA in mammalian cells at single-cell resolution. We extended this approach to observe DNA and RNA behaviors in several basic stages of cell division. We further demonstrated that the derivative can be used for fluorescence imaging of tumor in live mice.

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