2. MAPK/ERK Pathway Stem Cell/Wnt PI3K/Akt/mTOR
  3. ERK Akt
  4. NS-417 free base

NS-417 free base 是一种 ERK1、ERK2 及 Akt 激酶激活剂，具有神经保护、促进轴突生长和增强多巴胺能细胞群活性的功能。NS-417 free base 可通过生长因子刺激增强 ERK1、ERK2 和 Akt 激酶的激活。NS-417 free base 可在体外模型中挽救因生长因子缺失诱导死亡的细胞、刺激轴突生长、增加酪氨酸羟化酶阳性细胞计数，并表现出类似神经营养的活性。NS-417 free base 可用于帕金森病的研究。

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NS-417 free base

NS-417 free base Chemical Structure

CAS No. : 160753-58-2

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NS-417 free base 相关抗体

Top Publications Citing Use of Products

查看 ERK 亚型特异性产品:

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ERK ERK1 ERK2 ERK5 ERK8

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Akt Akt1 Akt2 Akt3

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

NS-417 free base is an ERK1, ERK2, and Akt kinase activator with neuroprotective, neurite outgrowth potentiating, and dopaminergic cell population enhancing activity. NS-417 free base enhances activation of ERK1, ERK2, and Akt kinase via growth factor stimulation. NS-417 free base rescues cells from growth factor withdrawal-induced death, stimulates neurite outgrowth, increases tyrosine hydroxylase-positive cell counts, and displays neurotrophic-like activity in in vitro models. NS-417 free base can be used for the research of parkinson’s disease[1].

IC50 & Target[1]

ERK1

 

ERK2

 

Akt

 

体外研究
(In Vitro)

NS-417 (0.01-10 μM；4 天) 可防止去除血清和 NGF 的分化大鼠 PC12 嗜铬细胞瘤细胞死亡，孵育 4 天后在 2-5 μM 浓度下观察到最大存活效应[1]。
NS-417 (1-3 μM；2 天) 可在未分化大鼠 PC12 嗜铬细胞瘤细胞中诱导短轴突样结构，并在孵育 2 天后显著增强由 NGF、bFGF 和 EGF 诱导的轴突生长[1]。
NS-417 (1-3 μM；24 小时) 可增强大鼠 PC12 嗜铬细胞瘤细胞中 NGF 诱导的长期 (24 小时) ERK1/ERK2 和 Akt 活化；且在 3 μM (10 min) 条件下，可增强 NGF、bFGF 和 EGF 诱导的短期 (10 分钟) ERK1/ERK2 活化[1]。
NS-417 (1 μM；7 天) 可使 E14 大鼠中脑腹侧分离培养物中酪氨酸羟化酶阳性多巴胺能神经元数量在孵育 7 天后增加 81%[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

NS-417 free base 相关抗体:

Cell Viability Assay[1]

Cell Line: differentiated rat PC12 pheochromocytoma cells
Concentration: 0.01 μM; 0.02 μM; 0.05 μM; 0.1 μM; 0.2 μM; 1 μM; 2 μM; 5 μM; 10 μM
Incubation Time: 4 days
Result: Prevented cell loss in a dose-dependent manner.
Exerted maximal survival effect at 2-5 μM, comparable to 3 nM NGF.
Resulted in significantly higher cell viability at 1, 2, 5, and 10 μM compared to untreated cells.
Preserved the neurites of surviving cells.

Western Blot Analysis[1]

Cell Line: rat PC12 pheochromocytoma cells
Concentration: 1 μM; 3 μM
Incubation Time: 24 h; 10 min
Result: Potentiated 24-hour NGF-induced activation of ERK1/ERK2 and phosphorylation of Akt (Ser-473).
Enhanced ERK1/ERK2 activation induced by 10 minutes of stimulation with NGF, bFGF, or EGF at 3 μM.
分子量

341.79

Formula

C18H16ClN3O2
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

NS-417 free base 相关分类

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  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NS-417160753-58-2NS417NS 417ERKAktExtracellular signal regulated kinasesPKBProtein kinase BNGFERK1tyrosine hydroxylase-positive cell countsE14 rat ventral mesencephalic culturesERK2parkinson’s diseasebFGFdopaminergic neuronsrat PC12 pheochromocytoma cellsAkt kinaseInhibitorinhibitorinhibit

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