2. Immunology/Inflammation Apoptosis GPCR/G Protein
  3. Macrophage migration inhibitory factor (MIF) Fc Receptor (FcR) TNF Receptor IFNAR CCR Interleukin Related
  4. ON203

ON203 是一种全人源 IgG1 抗氧化型巨噬细胞迁移抑制因子 (oxMIF) 的单克隆抗体，其 EC50 为 0.3 nM。ON203 可选择性结合 oxMIF，增强与 FcγRIIIA 和 FcγRIIA 的结合，触发抗体依赖的细胞介导的细胞毒性作用 (ADCC) 和抗体依赖的细胞吞噬作用 (ADCP)。ON203 可诱导 TNFα、IFNγ、MCP-1、IL6 释放，抑制肿瘤生长，降低肿瘤细胞增殖能力，减少肿瘤血管生成。ON203 可用于前列腺癌的研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

ON203

ON203 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
1 mg   询价  
5 mg   询价  
10 mg   询价  

* Please select Quantity before adding items.

Customer Review

ON203 相关抗体

Top Publications Citing Use of Products

查看 TNF Receptor 亚型特异性产品:

查看所有亚型
TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

查看 CCR 亚型特异性产品:

查看所有亚型
CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9

查看 Interleukin Related 亚型特异性产品:

查看所有亚型
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

ON203 is a fully human IgG1 monoclonal antibody targeting oxidized macrophage migration inhibitory factor (oxMIF), with an EC50 of 0.3 nM. ON203 selectively binds to oxMIF, enhances binding to FcγRIIIA and FcγRIIA, and triggers antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP). ON203 induces the release of TNFα, IFNγ, MCP-1, and IL6, inhibits tumor growth, reduces the proliferative capacity of tumor cells, and decreases tumor angiogenesis. ON203 can be used in the research of prostate cancer[1].

反应种属

Human
体外研究
(In Vitro)

ON203 (0.01-10 nM; 1 h) 在 ELISA 实验中对固定化人源 oxMIF 表现出 0.3 nM 的表观亲和力 (EC50)[1]。
ON203 可在溶液中结合人源 oxMIF，其 EC50 为 0.6 nM；结合小鼠源 oxMIF 的 EC50 为 5.2 nM，保持着物种特异性的低纳摩尔亲和力[1]。
ON203 可与可溶性人源 oxMIF 结合，其 KD 值为 6.1 nM；与可溶性小鼠源 oxMIF 结合的 KD 值为 31.6 nM[1]。
ON203 (0.01-100 nM; 6 h) 在报告基因实验中可强效诱导抗体依赖性细胞介导的细胞毒性，针对 FcγRIIIA V158 效应细胞的 EC50 值为 0.2-0.4 nM，针对 FcγRIIIA F158 效应细胞的 EC50 值为 0.4-0.7 nM[1]。
ON203 (0.01-100 nM; 6 h) 可在采用 FcγRIIA H131 效应细胞的报告基因实验中强效诱导抗体依赖性细胞吞噬作用[1]。
ON203 (0.01-100 nM; 过夜) 可诱导强效的 PBMC 介导的针对 HCT116/HiBiT/pMIF 细胞的 ADCC 效应，其 EC50 值为 0.1-0.7 nM[1]。
ON203 (0.07-70 nM; 24 h) 可诱导人外周血单个核细胞 (PBMC) 释放靶标依赖性促炎细胞因子 (TNFα、IFNγ、MCP-1、IL6)，且在无靶细胞的情况下未检测到非特异性细胞因子释放[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

ON203 相关抗体:
体内研究
(In Vivo)

ON203 (5-20 mg/kg；腹腔注射；每两天一次；共 15 次) 可在预防性小鼠异种移植模型中对前列腺肿瘤生长产生持久且显著的抑制作用，同时降低肿瘤细胞的增殖及血管生成[1]。
ON203 (20-60 mg/kg；腹腔注射；隔日一次；共 15 次) 在小鼠异种移植模型中对前列腺肿瘤生长的抑制作用最为显著，可使平均肿瘤体积减小 61%，并降低肿瘤细胞增殖活性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: NMRI nude (male, 6-9 weeks old, subcutaneous injection of 5×106 PC3 human prostate adenocarcinoma cells)[1]
Dosage: 5 mg/kg; 20 mg/kg
Administration: i.p.; every other day; 15 times
Result: Significantly inhibited tumor growth with durable response during post-treatment washout period at 20 mg/kg.
Showed no meaningful difference from controls at 5 mg/kg.
Reduced Ki67-positive cell count, tumor vascularization, and tumor cell intravasation into blood vessels at 20 mg/kg.
Maintained stable body weight across all groups with no acute toxicity observed.
Animal Model: NMRI nude (male, 6-9 weeks old, subcutaneous injection of 5×106 PC3 human prostate adenocarcinoma cells, treatment initiated when tumor volumes reached 70-110 mm3)[1]
Dosage: 20 mg/kg; 40 mg/kg; 60 mg/kg
Administration: i.p.; every other day; 15 times
Result: Reduced mean tumor volume by 61% on last treatment day at 40 mg/kg compared to vehicle/isotype control.
Reduced mean tumor volume by 45% at 60 mg/kg compared to vehicle/isotype control.
Reduced mean tumor volume by 10% at 20 mg/kg compared to vehicle/isotype control.
Decreased Ki67-positive cell count at 40 mg/kg and 60 mg/kg compared to controls.
Maintained stable body weight across all groups with no toxicity observed.
基因 ID

4282  [NCBI]

Accession
靶点

MIF
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
组分

Please refer to the lot-specific COA for specific buffer information.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

ON203 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ON203ON 203ON-203Macrophage migration inhibitory factor (MIF)Fc Receptor (FcR)TNF ReceptorIFNARCCRInterleukin RelatedFc ReceptorTumor Necrosis Factor ReceptorTNFRInterferon-α/β receptorInterferon-alpha/beta receptor CC chemokine receptorILADCCADCPoxMIFFcγRIIAIL6FcγRIIIAprostate cancerTNFαIFNγMCP-1Inhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
ON203
目录号：
HY-P992432
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z