2. Apoptosis Epigenetics Cell Cycle/DNA Damage
  3. Apoptosis PARP
  4. R9-caPep

R9-caPep (RRRRRRRRRCCLGIPEQEY) 是一种增殖细胞核抗原 (PCNA) 衍生的细胞穿透肽。R9-caPep 可选择性阻断 PCNA 与 FEN1 和 LIGI 的相互作用，同时保留 POLD3 与 PCNA 的结合。R9-caPep 可干扰 DNA 合成以及同源重组介导的双链 DNA 断裂修复，引发 S 期阻滞、DNA 损伤累积和细胞凋亡 (apoptosis)。R9-caPep 抑制裸鼠神经母细胞瘤的肿瘤体积与质量增长。R9-caPep 可用于神经母细胞瘤、三阴性乳腺癌的相关研究。

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Custom Peptide Synthesis

R9-caPep

R9-caPep Chemical Structure

CAS No. : 1207092-73-6

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R9-caPep 相关抗体

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

R9-caPep (RRRRRRRRRCCLGIPEQEY) is a cell-penetrating peptide derived from proliferating cell nuclear antigen (PCNA). R9-caPep selectively blocks the interactions between PCNA and FEN1, as well as between PCNA and LIGI, while preserving the binding of POLD3 to PCNA. R9-caPep interferes with DNA synthesis and homologous recombination-mediated double-strand DNA break repair, inducing S-phase arrest, DNA damage accumulation, and apoptosis. R9-caPep inhibits the growth of tumor volume and weight of neuroblastoma in nude mice. R9-caPep can be used in research related to neuroblastoma and triple-negative breast cancer[1][2].

体外研究
(In Vitro)

R9-caPep (72 h) 抑制多种神经母细胞瘤细胞系增殖， IC50 介于 10~32 mM，阻断增殖细胞核抗原 (PCNA) 的相互作用[1]。
R9-caPep (0-40 μM，72 h) 在 SK-N-BE(2)c 细胞中显著降低溴脱氧尿苷阳性细胞比例，造成细胞 DNA 复制停滞，抑制 SV40T 抗原介导的 DNA 复制，导致细胞发生 S 期周期阻滞，诱导细胞凋亡[1]。
R9-caPep (60 μM; 72 h) 可诱导大多数受试乳腺癌细胞系发生细胞死亡，其中对 HCC1428 细胞 (细胞死亡增加 49%) 和 MDA-MB-231 细胞 (细胞死亡增加 47%) 的作用最强，而对 BT474 细胞无显著影响[2]。
R9-caPep (75 μM，0-72 h) 可诱导 MDA-MB-231 细胞发生进行性 DNA 损伤和凋亡，自 24 h 起 γH2AX 水平持续升高，至 72 h 时超过 70% 的细胞出现 PARP 裂解[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

R9-caPep 相关抗体:

Cell Cycle Analysis[1]

Cell Line: SK-N-BE(2)c cells
Concentration: 0, 20, 40 μM
Incubation Time: 48 h
Result: Caused S-phase cell cycle arrest in neuroblastoma cells, and increased the proportion of cells in the sub-G1 phase.

Cell Viability Assay[2]

Cell Line: HCC1428, MDA-MB-231, BT474, MDA-MB-436, MCF7, SKBR3, MDA-MB-468, HCC1143, HCC1937, UACC893, HCC38, MDA-MB-361, HCC1569
Concentration: 60 μM
Incubation Time: 72 h
Result: Induced increased cell death relative to scrambled control in all tested breast cancer cell lines except BT474.
Increased cell death by 49% in HCC1428 cells.
Increased cell death by 47% in MDA-MB-231 cells.
Showed no significant cell death increase in BT474 cells.

Immunofluorescence[2]

Cell Line: MDA-MB-231
Concentration: 75 μM
Incubation Time: 12, 24, 48, 72 h
Result: Increased the population of cells with high γH2AX levels over the first 24 h, with levels remaining elevated through 72 h.
Increased the percentage of cells with high levels of cleaved PARP progressively over 72 h, rising from 0.2% at 0 h to over 70% by 72 h.
体内研究
(In Vivo)

R9-caPep (每周 3 次，瘤内注射) 抑制裸鼠异种移植模型中神经母细胞瘤的肿瘤体积与质量增长[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nude mice (6 weeks, SK-N-BE(2)c cells (5x107 xenografts)) [1]
Dosage: /
Administration: intratumoral injection, three times per week
Result: Inhibited the growth of tumor volume and mass of neuroblastoma in nude mouse xenograft models.
分子量

2560.02

Formula

C103H183N47O26S2
CAS 号
Sequence

Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Cys-Cys-Leu-Gly-Ile-Pro-Glu-Gln-Glu-Tyr
Sequence Shortening

RRRRRRRRRCCLGIPEQEY
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

R9-caPep 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

R9-caPep1207092-73-6RRRRRRRRRCCLGIPEQEYApoptosisPARPpoly ADP ribose polymeraseneuroblastomaFEN1PCNAhomologous recombination-mediated double-stranded DNA break repairPOLD3LIGIMDA-MB-231 cellstriple-negative breast cancer cellsDNA synthesiscisplatin-resistant breast cancercell-penetrating peptideInhibitorinhibitorinhibit

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