RADA 

RADA is a fluorescent D-amino acid (FDAA) with high photostability and thermostability, which emits yellow-to-orange fluorescence. RADA shows low outer membrane permeability in wild-type Gram-negative Escherichia coli, but it targets penicillin-binding proteins and L,D-transpeptidases, mimics the interaction between acyl acceptors and enzyme intermediates, and integrates into peptidoglycan during biosynthesis. As a peptidoglycan labeling reagent, RADA metabolically integrates into the nascent peptidoglycan of live bacterial cells, labels the peptidoglycan at the poles and lateral walls of mycobacteria, and enables visualization of peptidoglycan synthesis and remodeling processes. RADA serves as a non-specific stain for fixed cells, is non-toxic to bacterial cells, and its red-shifted excitation/emission spectra reduce phototoxicity. RADA also supports virtual pulse-chase labeling experiments and stochastic optical reconstruction microscopy for sub-diffraction-limited imaging of bacterial cell walls^[1].

RADA (25 μM; 15 min) 可标记耻垢分枝杆菌 (Mycobacterium smegmatis) 细胞的两极和侧壁，显示出伴随分枝菌膜代谢、呈不对称极性梯度分布的肽聚糖重塑过程^[1]。
RADA (25 μM; 15 min) 在耻垢分枝杆菌中的标记作用被 imipenem (HY-B1369A) 和 D-cycloserine (HY-B0030) 显著抑制，且其对 imipenem 的敏感性高于其他单肽 D-氨基酸探针^[1]。
RADA (25 μM; 15 min) 对耻垢分枝杆菌的标记显示，在生长过程中荧光稀释仅局限于菌体两极，说明细胞壁扩增受两极限制，且 RADA 高亮度极的生长速度更快^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

516.55

C₂₈H₂₈N₄O₆

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. García-Heredia A, et al. Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria. Elife. 2018;7:e37243. Published 2018 Sep 10.  [Content Brief]