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  4. Rhodamine-DHPE

Rhodamine-DHPE 是一种荧光标记的磷脂酰乙醇胺脂质，可标记磷脂双分子层。Rhodamine-DHPE 可作为荧光淬灭底物与膜染色剂。在 Cu2+-PS 复合物存在下，Rhodamine-DHPE 荧光寿命会显著降低。Rhodamine-DHPE 能够有效对人红细胞和小鼠成纤维细胞膜进行染色，并支持通过泵浦探测荧光显微镜实现寿命分辨成像。

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Rhodamine-DHPE

Rhodamine-DHPE Chemical Structure

CAS No. : 126111-99-7

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Rhodamine-DHPE 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Rhodamine-DHPE is a fluorescently labeled phosphatidylethanolamine lipid that labels phospholipid bilayers. Rhodamine-DHPE serves as a fluorescence quenching substrate and membrane stain. The fluorescence lifetime of Rhodamine-DHPE decreases significantly in the presence of Cu2+-PS complexes. Rhodamine-DHPE effectively stains the membranes of human red blood cells and mouse fibroblasts, and supports lifetime-resolved imaging via pump-probe fluorescence microscopy[1][2][3].

体外研究
(In Vitro)

Cu2+-PS 复合物可动态淬灭支撑脂质双层中 Rhodamine-DHPE 的荧光，荧光寿命缩短以及淬灭效率不随温度变化[2]。
Rhodamine-DHPE (5 mg/mL; 30 min) 用于人红细胞成像；与传统单光子显微镜相比，泵浦探测荧光显微镜在成像 Rhodamine-DHPE 标记的人红细胞时具有更出色的空间分辨率和焦外背景抑制能力[3]。
Rhodamine-DHPE (10 μg/mL; 30 min) 用于固定小鼠成纤维细胞的成像，其中泵浦探测荧光显微镜在成像 Rhodamine-DHPE 标记的固定小鼠成纤维细胞时，相比传统单光子显微镜具有更优异的空间分辨率[3]。
Rhodamine-DHPE (10 μg/mL; 30 min) 用于固定小鼠成纤维细胞的成像，在此过程中，时间分辨泵浦探测荧光显微镜可区分经 Rhodamine-DHPE 染色的细胞质结构与经溴化乙啶标记的固定小鼠成纤维细胞细胞核，从而在发射光谱重叠的区域间产生清晰的对比度[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Rhodamine-DHPE 相关抗体:
分子量

1333.80

Formula

C70H117N4O14PS2
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Rhodamine-DHPE 相关分类

  • 摩尔计算器

  • 稀释计算器

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rhodamine-DHPE126111-99-7Fluorescent Dyemembrane stainphosphatidylethanolamine lipidlipid bilayer markerhuman erythrocyteartificial high-cholesterol lipid vesicles3T3 fibroblast cell membrane-derived vesiclespump-probe fluorescence microscopyPOPC SLBmouse fibroblast cell membranesfluorescence quenching substrateInhibitorinhibitorinhibit

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目录号：
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