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  4. TMR-DN

TMR-DN (TAMRA-2,4-dinitroaniline) 是一种明亮的或橙色的可结合 SRB-2 适配体的荧光探针，对 SRB-2 适配体的 Kd 值为 35 nM。TMR-DN 具有低背景荧光，从而实现无需洗涤的活细胞 RNA 成像。

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TMR-DN

TMR-DN Chemical Structure

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TMR-DN 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

TMR-DN (TAMRA-2,4-dinitroaniline) is a bright orange fluorescent probe that binds to the SRB-2 aptamer, with a Kd value of 35 nM for the SRB-2 aptamer. TMR-DN exhibits low background fluorescence, enabling wash-free live-cell RNA imaging[1].

体内研究
(In Vivo)
Animal Model: Escherichia coli BL21 Star™ (DE3) (transformed with pET28-SRB-2 or pET28-tRNA plasmid, grown to OD600=0.4, induced with 1 mM IPTG for 2-3 h for high SRB-2 expression; grown without IPTG for low SRB-2 expression)[1]
Dosage: 500 nM (high SRB-2 expression imaging); 250 nM, 500 nM, 1 μM, 2 μM (low SRB-2 expression imaging)
Administration: incubation; 10 min (500 nM); 37°C
Result: Yielded a signal-to-background ratio of ~256 at 500 nM, which was 49-, 27-, and 9-fold higher than ratios for pyronin B, pyronin Y, and SR-DN respectively.
Showed ~11-fold higher fluorescence in SRB-2-expressing bacteria than SR-DN at 250 nM with high SRB-2 expression.
Yielded signal-to-background ratios of 2.9 (1 μM), 3.6 (500 nM), and 11.2 (250 nM) for low SRB-2 expression, while SR-DN failed to produce distinguishable signal.
Enabled imaging of GFP mRNA fused to 6xSRB-2 or 15xSRB-2, with 15xSRB-2-expressing bacteria showing 2.4-fold brighter fluorescence than 6xSRB-2-expressing bacteria.
Animal Model: HeLa (DSMZ, ACC 57) (seeded to 60-80% confluency, co-transfected with pcDNA-CFP and pAV-5S-SRB-2 or pAV-5S control plasmid; or transfected with CFP-TM-15xSRB-2 or CFP-TM control plasmid, incubated for 24-36 h)[1]
Dosage: 500 nM
Administration: incubation; 30 min; 37°C
Result: Enabled clear visualization of the nuclear and cytoplasmic distribution of 5S rRNA tagged with SRB-2, with minimal background fluorescence in control cells expressing untagged 5S rRNA.
Showed significantly higher fluorescence in HeLa cells expressing CFP-TM mRNA tagged with 15xSRB-2 than control cells, with cytoplasmic localization of the mRNA confirmed; northern blot analysis verified the signal originated from full-length transcript, not degradation products.
分子量

726.73

Formula

C37H38N6O10
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

TMR-DN 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TMR-DNTAMRA-2,4-dinitroanilineFluorescent Dyetotal RNASRB-2 aptamermessenger RNAmammalian cellsbacterial cellsribosomal RNA5S rRNAGFP mRNABL21 Star? (DE3) E. coliHeLa cellsInhibitorinhibitorinhibit

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产品名称：
TMR-DN
目录号：
HY-D3416
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