磷脂酶A2 来源于东部菱背响尾蛇毒液

别名 PLA2;Lecithinase A;Phosphatidylcholine 2-acylhydrolase
英文别名 PLA2;Lecithinase A;Phosphatidylcholine 2-acylhydrolase
规格或纯度 ≥200 units/mg dry weight
英文名称 Phospholipase A2 from Crotalus adamanteus Venom 单位定义 One Unit releases one micromole of acid from soybean lecithin per minute at 25°C, pH 8.9 生化机理 Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells. It has a high catalytic activity on aggregated substrates compared to monomeric substrates.
储存温度 2-8°C储存
运输条件 冰袋运输
产品介绍 Phospholipase A2 is a member of the class of heat-stable, calcium-dependent enzymes catalyzing the hydrolysis of the 2-acyl bond of 3-n-phosphoglycerides. The enzyme has a molecular weight of 30,000 daltons. Phospholipase A2 is activated by Ca2+. It is inhibited by zinc, barium, and manganese ions. Activity values for phospholipase A preparations which are derived from titrimetric assay procedures can be quite dependent on source and type of lecithin, its preparation as a substrate emulsion, other components of the reaction mixture, and the method and instrumentation used. A chromatographically purified, dialyzed, lyophilized powder.