1. 抗体
  2. 一抗
  3. 多克隆抗体
  4. Connexin 43 抗体

Connexin 43 Antibody 是一个兔来源、无偶联标记、抗 Connexin 43 的 IgG 多克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
20 μL ¥738 In-stock
50 μL ¥1060 In-stock
100 μL ¥1700 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Connexin 43 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Connexin 43.

宿主

Rabbit

克隆性

Polyclonal

分子量
Predicted band size: 43 kDa;
Observed band size: 43 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human Connexin 43.AA range:333-382

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
IHC-F
IHC-F: 冰冻切片样本的免疫组织化学
1:50-1:100
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
ELISA
ELISA: 酶联免疫吸附试验
1:10000
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Connexin 43 Antibody (HY-P80628) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Connexin 43 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Connexin 43 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:间隙连接蛋白,作为膀胱容量的调节因子。间隙连接由紧密排列的跨膜通道 (连接子) 组成,低分子量物质可通过这些通道从一个细胞扩散到相邻细胞。它可能通过参与钾离子向耳蜗内淋巴的循环,在听觉生理中发挥关键作用。膀胱功能容量的负调控因子:通过增强细胞间电信号和化学信号传递,使膀胱肌肉对胆碱能神经刺激更加敏感,从而引起收缩 (基于相似性)。可能通过调节 NOV 的表达和定位,在细胞生长抑制中发挥作用。在脑室的间隙连接通讯中起着至关重要的作用 (基于相似性)。
亚细胞定位:细胞膜;多通道膜蛋白;细胞连接,间隙连接;内质网
表达水平:
组织特异性:在心脏闰盘中表达 (蛋白质水平) (PubMed:11741837,PubMed:18662195) 。在胎儿耳蜗中表达 (PubMed:11741837) 。
亚基:连接子由六聚体连接蛋白组成。通过 C 端与 TJP1 相互作用 (基于相似性)。通过 C 端与 SRC 相互作用 (通过 SH3 结构域)(基于相似性)。
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
Connexin 43 抗体
同用名
Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1;
文件资料

Connexin 43 Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Connexin 43 Antibody
目录号:
HY-P80628
需求量: