1. 抗体
  2. 一抗
  3. 单克隆抗体
  4. Hsp70 抗体 (YA728)

Hsp70 Antibody (YA728) 是一个小鼠来源、无偶联标记、抗 Hsp70 的单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥ 491.40

¥756.00

In-stock
50 μL ¥ 1296.75

¥1995.00

In-stock
100 μL ¥ 2047.50

¥3150.00

In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Hsp70 Antibody (YA728) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Hsp70.

宿主

Mouse

克隆性

Monoclonal

分子量
Predicted band size: 70 kDa;
Observed band size: 70 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic Peptide of HSP70

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:1000-1:2000
ICC/IF
ICC/IF: 细胞免疫荧光
1:100-200
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-300
敏感性 Endogenous 纯度 Immunogen affinity purified.
偶联 Non-conjugated 修饰 Unmodified
性状

液体

组分

Supplied in 1*PBS ( pH 7.4) and 50% Glycerol. Preservative: 0.02% sodium azide

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ICC
  • Immunocytochemistry analysis of HeLa cells labeling HSP70 Antibody (HY-P80412) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSP70 Antibody (HY-P80412) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of NIH/3T3 cells labeling HSP70 Antibody (HY-P80412) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with HSP70 Antibody (HY-P80412) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:分子伴侣参与多种细胞过程,包括保护蛋白质组免受应激、折叠和运输新合成的多肽、激活错误折叠蛋白质的蛋白水解以及蛋白质复合物的形成和解离。它在蛋白质质量控制系统中发挥着关键作用,确保蛋白质的正确折叠、错误折叠蛋白质的重新折叠以及控制蛋白质的靶向降解。这一过程是通过 ATP 结合、ATP 水解和 ADP 释放的循环实现的,并由辅助伴侣介导。其对多肽的亲和力受其核苷酸结合状态的调控。在 ATP 结合状态下,它对底物蛋白的亲和力较低。然而,当 ATP 水解为 ADP 后,它发生构象变化,从而提高其对底物蛋白的亲和力。它经历反复的 ATP 水解和核苷酸交换循环,从而实现底物结合和释放的循环 (PubMed:26865365)。 PRKN 转位至受损线粒体的正调控因子 (PubMed:24270810)
表达水平:
组织特异性:在精子细胞中表达

诱导:并非由热休克引起
亚基:与 PRKN 相互作用
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
Hsp70 抗体 (YA728)
文件资料

Hsp70 Antibody (YA728) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
Hsp70 Antibody (YA728)
目录号:
HY-P80412
需求量: