RhoA/B/C 抗体

目录号: HY-P80882 一键复制产品信息: Data Sheet SDS COA 抗体使用指南
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技术支持

RhoA/B/C Antibody 是一个兔来源、无偶联标记、抗 RhoA/B/C 的 IgG 多克隆抗体。

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规格	价格	是否有货
10 μL	￥493	In-stock
50 μL	￥1281	In-stock
100 μL	￥2100	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

产品详情
验证图片
背景信息
产品资料

描述

RhoA/B/C Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to RhoA/B/C.

宿主

Rabbit

克隆性

Polyclonal

分子量

Predicted band size: 22 kDa;

Observed band size: 22 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

P61586 / P62745 / P08134

基因 ID

387 [NCBI]

免疫原

Synthetic peptide corresponding to Human Rho A + B + C.The exact sequence is proprietary to MCE.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:100
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
FC FC: 流式细胞术	1:50-1:100

敏感性	Endogenous	纯度	affinity purified
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL WB ICC IHC-P FC

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) ,HepG2(lane 3(20ug) and Hela(lane 4(20ug) using RhoA/B/C Antibody (HY-P80882) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HepG2 cells labeling RhoA/B/C with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH3T3 cells labeling RhoA/B/C with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with RhoA/B/C Antibody (HY-P80882) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Collagen VI alpha 123 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using Collagen VI alpha 123 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of 1X10^6 NIH-3T3 cells labeling RhoA/B/C Antibody (HY-P80882, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

背景

功能：P61586：一种小型 GTP 酶，在活性 GTP 结合态和非活性 GDP 结合态之间循环。主要与细胞骨架组织相关，在活性状态下与多种效应蛋白结合，调节细胞反应，例如细胞骨架动力学、细胞迁移和细胞周期 (PubMed:23871831)。调节连接质膜受体与黏着斑和肌动蛋白应力纤维组装的信号转导通路 (PubMed:31570889, PubMed:8910519, PubMed:9121475)。参与微管依赖性信号传导，该信号传导是细胞周期胞质分裂过程中肌球蛋白收缩环形成所必需的 (PubMed:12900402, PubMed:16236794)。在细胞分裂沟形成中发挥重要作用。角质形成细胞间黏附的顶端连接形成所必需 (PubMed:20974804, PubMed:23940119)。SPATA13介导的细胞迁移和黏附组装与解离调控至关重要 (PubMed:19934221)。
P62745：在 DNA 损伤后介导肿瘤转化细胞的凋亡。虽然并非发育所必需，但会影响转化细胞的细胞黏附和生长因子信号传导。在肿瘤发生中起负调控作用，因为其缺失会导致肿瘤形成。参与多种蛋白质的细胞内运输。将 PKN1 靶向内体，并参与 EGF 受体从晚期内体到溶酶体的运输。也是 AKT1/AKT 稳定性及核转运所必需的，AKT1/AKT 在血管发育过程中促进内皮细胞存活。作为一种微管依赖性信号，是细胞周期胞质分裂过程中肌球蛋白收缩环形成所必需的。是乳腺癌细胞中基因毒性应激诱导的细胞死亡所必需的。
P08134：调控连接质膜受体与黏着斑和肌动蛋白应力纤维组装的信号转导通路。作为一种微管依赖性信号，是细胞周期胞质分裂过程中肌球蛋白收缩环形成所必需的。调控支气管上皮细胞顶端连接的形成。

亚细胞定位：P61586：细胞膜；脂锚；细胞质侧；细胞质，细胞骨架；分裂沟；细胞质，细胞皮层；中体；细胞突起，片状伪足；细胞突起，树突；细胞核；细胞质
P62745：晚期内体膜；脂锚；细胞膜；脂锚；细胞核；分裂沟
P08134：细胞膜；脂锚；胞质侧；分裂沟

表达水平：诱导：P62745：受 DNA 损伤因子 (如 H2O2 或电离辐射 (IR)) 上调

亚基：P61586：与 ARHGEF28 相互作用 (基于相似性)。通过 GTP 结合形式与 RIPOR1 (通过 N 端) 相互作用；这种相互作用将 RHOA 与 STK24 和 STK26 激酶连接起来 (PubMed:27807006)。
P62745：结合 ROCK1 和 ROCK2 (基于序列相似性)。也结合 PKN1/PRK1 (PubMed:9478917)。与 ARGGEF3 相互作用 (PubMed:12221096)。与 RTKN 相互作用 (基于序列相似性)。与 AKAP13 相互作用 (PubMed:11546812)。与 RIPOR1 相互作用 (PubMed:27807006)。
P08134：与 RTKN 相互作用 (基于相似性)。与 AKAP13 相互作用 (PubMed：11546812)。与 DIAPH1 相互作用 (PubMed：15864301)。与 PKN2 相互作用 (PubMed：20974804)。与 ROCK1 和 ROCK2 相互作用 (PubMed：8816443)。与 ARHGDIA 相互作用 (PubMed：20400958)。与 RIPOR1 相互作用 (PubMed：27807006)。

RRID

AB_3102989

反应种属数据库

Entrez Gene: 387 Human ; 388 Human ; 389 Human ; 11848 Mouse ; 11852 Mouse ; 11853 Mouse ; 117273 Rat 295342

SwissProt: P08134 Human ; P61586 Human ; P62745 Human ; P62746 Mouse ; Q62159 Mouse ; Q9QUI0 Mouse ; P61589 Rat P62747

OMIM: 165380 Human

研究领域

Signal Transduction

中文名

RhoA/B/C 抗体

同用名

ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (192 KB)

抗体使用指南 (1083 KB)

RhoA/B/C Antibody 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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