1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. SP7/Osterix 抗体 (YA3539)

SP7/Osterix Antibody (YA3539) 是一个兔来源、无偶联标记、抗 SP7/Osterix 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
20 μL ¥846 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

SP7/Osterix Antibody (YA3539) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SP7/Osterix.

宿主

Rabbit

克隆性

Monoclonal,Recombinant

分子量
Predicted band size: 45 kDa;
Observed band size: 45 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Recombinant protein within human Sp7 aa 1-300.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:2000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:200-1:1000
IF-Tissue
IF-Tissue: 组织免疫荧光
1:500
IHC-F
IHC-F: 冰冻切片样本的免疫组织化学
1:200
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from Saos-2 (lane 2(20μg) , Saos-2 (lane 3(40μg) ,using SP7/Osterix Antibody (HY-P81125). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue using SP7/Osterix Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P81125, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:成骨细胞分化所必需的转录激活因子 (PubMed:23457570)。与 SP1 和 EKLF 共识序列以及其他富含 G/C 的序列结合 (基于相似性)。
亚细胞定位:
表达水平:
组织特异性:仅限于骨源性细胞
阳性样本:Saos-2 cell lysates, human osteosarcoma tissue, mouse embryo tissue, rat embryo tissue, E14.5 mouse embryo tissue.
异构体 & 翻译后修饰:Q8TDD2 有两种异构体:Q8TDD2-1:44994 Da (预测值);Q8TDD2-2:43092 Da (预测值)。
泛素化导致蛋白酶体降解。SP7 是一种短寿命蛋白,内源性半衰期约为 12 小时;丙酰化。SIRT7 对 Lys-371 的去丙酰化作用激活转录因子活性,并正向调控成骨细胞的骨形成。
亚基:与 RIOX1 相互作用;这种相互作用是直接的,并抑制转录激活因子活性。
RRID
反应种属数据库
中文名
成骨相关转录因子抗体
同用名
MGC126598 antibody; Osterix antibody; Osx antibody; Sp 7 antibody; SP7 antibody; Sp7 transcription factor antibody; SP7_HUMAN antibody; Transcription factor Sp7 antibody; Zinc finger protein osterix antibody;
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
SP7/Osterix Antibody (YA3539)
目录号:
HY-P81125
需求量: