Cyanamide  (Synonyms: 氰胺)

Cyanamide is a cell division and plant growth inhibitor, as well as an allelochemical derived from Vicia villosa. Cyanamide inhibits root growth and biomass accumulation in a dose-dependent manner by disrupting the formation of mitotic spindles and phragmoplast complexes, reducing the number of mitotic cells and blocking the cell cycle. The effects of Cyanamide are partially reversible after removal from low-concentration environments. Cyanamide is also a specific inhibitor of aldehyde dehydrogenase (ALDH). Although Cyanamide has no direct effect on tumor growth, it can significantly enhance the anti-tumor efficacy of Cyclophosphamide (HY-17420) at non-toxic doses by inhibiting the inactivation of Cyclophosphamide. Cyanamide enables Cyclophosphamide to exert equivalent therapeutic effects at lower doses, effectively inhibiting the growth of primary and metastatic tumors and prolonging the lifespan of tumor-bearing mice. Cyanamide is commonly used in studies related to ha-1 hepatoma and rls lymphosarcoma^[1][2].

Cyanamide (2-10 mM; 1-6 d) 可剂量依赖性地抑制洋葱 (Allium cepa L.) 鳞茎根的根生长、鲜重积累、干重积累及 RGR，仅在低浓度 (2 mM) 处理后观察到生长完全恢复，高浓度处理下则仅部分恢复或完全无法恢复^[1]。
Cyanamide (2-10 mM; 1, 6 d) 可通过剂量和时间依赖性方式提高 H₂O₂ 浓度，诱导洋葱 (Allium cepa L.) 鳞茎根产生氧化应激，其中最高浓度 (10 mM) 处理 1 天后会引发 H₂O₂ 的急剧升高^[1]。
Cyanamide (2-10 mM; 1-6 d) 会改变洋葱鳞茎根中超氧阴离子的积累，其中 10 mM 处理 1 天后会导致超氧阴离子广泛积累，并随后引发组织降解^[1]。
Cyanamide (2-10 mM; 1-6 d) 会改变洋葱根尖细胞的有丝分裂指数，破坏染色体形态与中期板形成，且移除该化合物后，所有有丝分裂活性和染色体结构均可完全恢复^[1]。
Cyanamide (2-10 mM; 1-6 d) 会改变洋葱根尖细胞的细胞周期分布，在短期处理后提高 4C 细胞核的比例，并在恢复阶段提高 G₂/G₁ 比值^[1]。
Cyanamide (2-10 mM; 1 d) 会在 6 mM 和 10 mM 浓度下破坏洋葱根尖分生组织细胞的微管细胞骨架排列，而 2 mM 浓度对微管结构无可见影响^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cyanamide (70 mg/kg；腹腔注射；提前 Cyclophosphamide (HY-17420) 15 分钟处理) 可增强环磷酰胺 (Cyclophosphamide) 在小鼠肝细胞癌和淋巴肉瘤模型中的抗肿瘤功效，在肝细胞癌模型中可使动物寿命延长至少 80%，在淋巴肉瘤模型中可将环磷酰胺剂量降低 2/3 同时维持等效治疗效果^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

42.04

CH₂N₂

420-04-2

液体（密度：1.282 g/cm³）

氰胺

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Soltys D, et al. Cyanamide mode of action during inhibition of onion (Allium cepa L.) root growth involves disturbances in cell division and cytoskeleton formation. Planta. 2011;234(3):609-621.  [Content Brief]

  [2]. Kaledin V I, et al. Cyanamide promotes the antitumor effect of cyclophosphamide more than its toxic effect[C]//Doklady Biological Sciences. Springer Nature BV, 2008, 420(1): 195.