2. Academic Validation
  3. In Vitro Evaluation of Antiviral Activities of Funicone-like Compounds Vermistatin and Penisimplicissin against Canine Coronavirus Infection

In Vitro Evaluation of Antiviral Activities of Funicone-like Compounds Vermistatin and Penisimplicissin against Canine Coronavirus Infection

  • Antibiotics (Basel). 2023 Aug 15;12(8):1319. doi: 10.3390/antibiotics12081319.
Claudia Cerracchio 1,  Maria Michela Salvatore 2 3,  Luca Del Sorbo 1,  Francesco Serra 4,  Maria Grazia Amoroso 4,  Marina DellaGreca 2,  Rosario Nicoletti 5 6,  Anna Andolfi 2 7,  Filomena Fiorito 1 7
Affiliations

Affiliations

  • 1 Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy.
  • 2 Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy.
  • 3 Institute for Sustainable Plant Protection, National Research Council, 80055 Portici, Italy.
  • 4 Istituto Zooprofilattico Sperimentale del Mezzogiorno, Unit of Virology, Department of Animal Health, 80055 Portici, Italy.
  • 5 Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy.
  • 6 Council for Agricultural Research and Economics, Research Centre for Olive, Fruit and Citrus Crops, 81100 Caserta, Italy.
  • 7 BAT Center-Interuniversity Center for Studies on Bioinspired Agro-Environmental Technology, University of Naples Federico II, 80055 Portici, Italy.
PMID: 37627739 DOI: 10.3390/antibiotics12081319
Abstract

Recent studies have demonstrated that 3-O-methylfunicone (OMF), a Fungal secondary metabolite from Talaromyces pinophilus belonging to the class of funicone-like compounds, has Antiviral activity against canine coronaviruses (CCoV), which causes enteritis in dogs. Herein, we selected two additional funicone-like compounds named vermistatin (VER) and penisimplicissin (PS) and investigated their inhibitory activity towards CCoV Infection. Thus, both compounds have been tested for their cytotoxicity and for Antiviral activity against CCoV in A72 cells, a fibrosarcoma cell line suitable for investigating CCoV. Our findings showed an increase in cell viability, with an improvement of morphological features in CCoV-infected cells at the non-toxic doses of 1 μM for VER and 0.5 μM for PS. In addition, we observed that these compounds caused a strong inhibition in the expression of the Aryl Hydrocarbon Receptor (AhR), a ligand-activated transcription factor which is activated during CCoV Infection. Our results also showed the alkalinization of lysosomes in the presence of VER or PS, which may be involved in the observed Antiviral activities.

Keywords

A72 cells; Talaromyces pinophilus; aryl hydrocarbon receptor; canine coronavirus; lysosome acidity; secondary fungal metabolites; viral nucleocapsid protein; virus yield.

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